Distinct expression profiles of regulatory RNAs in the response to biocides in Staphylococcus aureus and Enterococcus faecium

Loren Dejoies (1, 2) , Killian Le Neindre (1, 2) , Sophie Reissier (2, 3) , Brice Felden (2) , Vincent Cattoir (1, 2, 4)

1 Centre Hospitalier Universitaire de Rennes [CHU Rennes] = Rennes University Hospital [Ponchaillou]
2 BRM - ARN régulateurs bactériens et médecine
3 AGIR - Agents infectieux, résistance et chimiothérapie - UR UPJV 4294
4 National Reference Center for Antimicrobial Resistance (lab Enterococci)

The aim of the study was to characterize the antimicrobial activity of clinically-relevant biocides (chlorhexidine digluconate, benzalkonium chloride, PVP-iodine and triclosan) and to determine the sRNA expression profiles under biocide exposure in two major bacterial opportunistic pathogens, Enterococcus faecium and Staphylococcus aureus. In vitro activities were evaluated against S. aureus HG003 and E. faecium Aus0004. We determined MIC, MBC, sub-inhibitory concentrations (SIC) and growth curves under SIC conditions. sRNA expression study under SIC exposure of biocides was performed by RT-qPCR on 3 sRNAs expressed in S. aureus (RNAIII, SprD and SprX) and the first 9 sRNAs identified as expressed in E. faecium. MICs were higher against E. faecium than for S. aureus. Growth curves under increasing biocide concentrations highlighted two types of bactericidal activity: "on/off" effect for chlorhexidine, benzalkonium chloride, PVP-iodine and a "concentration-dependent" activity for triclosan. Exposure to biocide SICs led to an alteration of several sRNA expression profiles, mostly repressed. The distinct biocide activity profiles must be evaluated with other compounds and bacterial species to enrich the prediction of resistance risks associated with biocide usage. Biocide exposure induces various sRNA-mediated responses in both S. aureus and E. faecium, and further investigations are needed to decipher sRNA-driven regulatory networks.

Mots clés: Antimicrobial resistance; Small RNAs; Bacterial genetics

Domaines: Sciences du Vivant [q-bio]

Loren Dejoies, Killian Le Neindre, Sophie Reissier, Brice Felden, Vincent Cattoir. Distinct expression profiles of regulatory RNAs in the response to biocides in Staphylococcus aureus and Enterococcus faecium. Scientific Reports, 2021, 11 (1), pp.6892. ⟨10.1038/s41598-021-86376-y⟩. ⟨hal-03245874⟩