Accumulation of free and covalently bound microcystins in tissues of Lymnaea stagnalis (Gastropoda) following toxic cyanobacteria or dissolved microcystin-LR exposure.

Emilie Lance (1) , Milla Riina Neffling (2) , Claudia Gerard (1) , Jussi Meriluoto (2) , Myriam Bormans (1)

1 ECOBIO - Ecosystèmes, biodiversité, évolution [Rennes]
2 Åbo Academy University

Accumulation of free microcystins (MCs) in freshwater gastropods has been demonstrated but accumulation of MCs covalently bound to tissues has never been considered so far. Here, we follow the accumulation of total (free and bound) MCs in Lymnaea stagnalis exposed to i) dissolved MC-LR (33 and 100 μg L−1) and ii) Planktothrix agardhii suspensions producing 5 and 33 μg MC-LR equivalents L−1 over a 5-week period, and after a 3-week depuration period. Snails exposed to dissolved MC-LR accumulated up to 0.26 μg total MCs g−1 dry weight (DW), with no detection of bound MCs. Snails exposed to MCs producing P. agardhii accumulated up to 69.9 μg total MCs g−1 DW, of which from 17.7 to 66.7% were bound. After depuration, up to 15.3 μg g−1 DW of bound MCs were detected in snails previously exposed to toxic cyanobacteria, representing a potential source of MCs transfer through the food web. The study concerns accumulation and elimination of both free and bound microcystins (MCs) in tissues of a gastropod exposed to MCs producing cyanobacteria or dissolved MC-LR.

Mots clés: Cyanobacteria; Gastropods; Covalently bound microcystins; Accumulation; Detoxification

Domaines: Biodiversité et Ecologie

Emilie Lance, Milla Riina Neffling, Claudia Gerard, Jussi Meriluoto, Myriam Bormans. Accumulation of free and covalently bound microcystins in tissues of Lymnaea stagnalis (Gastropoda) following toxic cyanobacteria or dissolved microcystin-LR exposure.. Environmental Pollution, 2010, 158 (3), pp.674-680. ⟨10.1016/j.envpol.2009.10.025⟩. ⟨hal-00448279⟩