Detection of free and covalently bound microcystins in animal tissues by liquid chromatography-tandem mass spectrometry. - Université de Rennes Accéder directement au contenu
Article Dans Une Revue Environmental Pollution Année : 2010

Detection of free and covalently bound microcystins in animal tissues by liquid chromatography-tandem mass spectrometry.

Résumé

Microcystins are cyanobacterial hepatotoxins capable of accumulation into animal tissues. The toxins act by inhibiting specific protein phosphatases and both non-covalent and covalent interactions occur. The 2-methyl-3-methoxy-4-phenylbutyric acid (MMPB) method determines the total, i.e. the sum of free and protein-bound microcystin in tissues. The aim of the method development in this paper was to tackle the problems with the MMPB methodology: the rather laborious workflow and the loss of material during different steps of the method. In the optimised workflow the oxidation recovery was of acceptable level (29-40%), the extraction efficiency good (62-97%), but the signal suppression effect from the matrix remained severe in our system (16-37% signal left). The extraction efficiency for the determination of the free, extractable microcystins, was found to be good, 52-100%, depending on the sample and the toxin variant and concentration.

Dates et versions

hal-00474702 , version 1 (20-04-2010)

Identifiants

Citer

Emilie Lance, Milla Riina Neffling, Claudia Gerard, Jussi Meriluoto, Myriam Bormans. Detection of free and covalently bound microcystins in animal tissues by liquid chromatography-tandem mass spectrometry.. Environmental Pollution, 2010, 158 (3), pp.948-952. ⟨10.1016/j.envpol.2009.10.023⟩. ⟨hal-00474702⟩
104 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More