A robust collagen scoring method for human liver fibrosis by second harmonic microscopy. - Université de Rennes Accéder directement au contenu
Article Dans Une Revue Optics Express Année : 2010

A robust collagen scoring method for human liver fibrosis by second harmonic microscopy.

Résumé

Second Harmonic Generation (SHG) microscopy offers the opportunity to image collagen of type I without staining. We recently showed that a simple scoring method, based on SHG images of histological human liver biopsies, correlates well with the Metavir assessment of fibrosis level (Gailhouste et al., J. Hepatol., 2010). In this article, we present a detailed study of this new scoring method with two different objective lenses. By using measurements of the objectives point spread functions and of the photomultiplier gain, and a simple model of the SHG intensity, we show that our scoring method, applied to human liver biopsies, is robust to the objective's numerical aperture (NA) for low NA, the choice of the reference sample and laser power, and the spatial sampling rate. The simplicity and robustness of our collagen scoring method may open new opportunities in the quantification of collagen content in different organs, which is of main importance in providing diagnostic information and evaluation of therapeutic efficiency.
Fichier principal
Vignette du fichier
oe-18-25-25794.pdf (1.7 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-00684532 , version 1 (01-10-2013)

Identifiants

Citer

Thomas Guilbert, Christophe Odin, Yann Le Grand, Luc Gailhouste, Bruno Turlin, et al.. A robust collagen scoring method for human liver fibrosis by second harmonic microscopy.. Optics Express, 2010, 18 (25), pp.25794-807. ⟨10.1364/OE.18.025794⟩. ⟨hal-00684532⟩
257 Consultations
258 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More