Theoretical and Experimental SHG Angular Intensity Patterns from Healthy and Proteolysed Muscles.

Denis Rouède (1) , Jean-Jacques Bellanger (2) , Emmanuel Schaub (1) , Gaëlle Recher (3) , François Tiaho (4)

1 IPR - Institut de Physique de Rennes
2 LTSI - Laboratoire Traitement du Signal et de l'Image
3 N&eD - Neurobiologie et Développement
4 Irset - Institut de recherche en santé, environnement et travail

SHG angular intensity pattern (SHG-AIP) of healthy and proteolysed muscle tissues are simulated and imaged here for the first time to our knowledge. The role of the spatial distribution of second-order nonlinear emitters on SHG-AIP is highlighted. SHG-AIP with two symmetrical spots is found to be a signature of healthy muscle whereas SHG-AIP with one centered spot in pathological mdx muscle is found to be a signature of myofibrillar disorder. We also show that SHG-AIP provides information on the three-dimensional structural organization of myofibrils in physiological and proteolysed muscle. Our results open an avenue for future studies aimed at unraveling more complex physiological and pathological fibrillar tissues organization.

Domaines: Biophysique; Ingénierie biomédicale

Denis Rouède, Jean-Jacques Bellanger, Emmanuel Schaub, Gaëlle Recher, François Tiaho. Theoretical and Experimental SHG Angular Intensity Patterns from Healthy and Proteolysed Muscles.. Biophysical Journal, 2013, 104 (9), pp.1959-68. ⟨10.1016/j.bpj.2013.02.047⟩. ⟨hal-00825645⟩