In vitro establishment and multiplication of the Normania triphylla (Lowe) Lowe

David Delmail 1, * Pascal Labrousse 2 Jean-Yves Lesouëf 3 François Le Hir 3 Jean-Luc Autret 3 Michel Botineau 2
* Corresponding author
1 ISCR - Institut des Sciences Chimiques de Rennes
2 GRESE - Groupement de Recherche Eau, Sol, Environnement
3 CBN - Conservatoire Botanique National de Brest
Abstract : Normania triphylla is an endemic species from Madeira island (Portugal) extinct in the wild since 1991. The aim of this work was to culture the meristems of this species in vitro and to multiply its shoots in order to preserve this endangered species. The best results in terms of multiplication were obtained in Murashige and Skoog medium supplemented with 10 µM 6‑benzylaminopurine (BAP). The number of shoots, the number of nodes and the number of leaves were the most important in this medium. However, the best results concerning the total shoot length were obtained when BAP was not supplemented into the medium and in the presence of 5 or 7.5 µM 1-naphtalene acetic acid (NAA). This is the first report on the in vitro culture of N. triphylla which could brings new avenues for the development of this species.
keyword : Normania Endemic species Micropropagation 6‑benzylaminopurine Callus
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Journal articles
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https://hal-univ-rennes1.archives-ouvertes.fr/hal-00834179
Contributor : David Delmail <>
Submitted on : Friday, June 14, 2013 - 1:38:25 PM
Last modification on : Saturday, December 22, 2018 - 7:21:40 PM

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UNIV-RENNES1 | UNILIM | GRESE | ISCR | INC-CNRS | UR1-UFR-SPM | ISCR-CORINT | PEIRENE | UNIV-RENNES | INSA-RENNES | GEIST | INSA-GROUPE

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David Delmail, Pascal Labrousse, Jean-Yves Lesouëf, François Le Hir, Jean-Luc Autret, et al.. In vitro establishment and multiplication of the Normania triphylla (Lowe) Lowe. Brazilian Archives of Biology and Technology, Tecpar, 2012, 55 (4), pp.543-547. ⟨10.1590/S1516-89132012000400009⟩. ⟨hal-00834179⟩

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