Light-induced reactivation of O2-tolerant membrane-bound [Ni-Fe] hydrogenase from the hyperthermophilic bacterium Aquifex aeolicus under turnover conditions. - Archive ouverte HAL Access content directly
Journal Articles Physical Chemistry Chemical Physics Year : 2013

Light-induced reactivation of O2-tolerant membrane-bound [Ni-Fe] hydrogenase from the hyperthermophilic bacterium Aquifex aeolicus under turnover conditions.

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Alexandre Ciaccafava
Cyrille Hamon
Pascale Infossi
  • Function : Author
Valérie Marchi
Elisabeth Lojou
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Abstract

We report the effect of UV-Vis light on the membrane-bound [Ni-Fe] hydrogenase from Aquifex aeolicus under turnover conditions. Using electrochemistry, we show a potential dependent light sensitivity and propose that a light-induced structural change of the [Ni-Fe] active site is related to an enhanced reactivation of the hydrogenase under illumination at high potentials.
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hal-00860457 , version 1 (10-09-2013)

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Alexandre Ciaccafava, Cyrille Hamon, Pascale Infossi, Valérie Marchi, Marie-Thérèse Giudici-Orticoni, et al.. Light-induced reactivation of O2-tolerant membrane-bound [Ni-Fe] hydrogenase from the hyperthermophilic bacterium Aquifex aeolicus under turnover conditions.. Physical Chemistry Chemical Physics, 2013, 15 (39), pp.16463-16467. ⟨10.1039/c3cp52596a⟩. ⟨hal-00860457⟩
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