Simultaneous determination of 12 beta-lactam antibiotics in human plasma by high-performance liquid chromatography with UV detection: application to therapeutic drug monitoring.

Marie-Clémence Verdier (1) , Olivier Tribut (1) , Pierre Tattevin (2) , Yves Le Tulzo (3, 4) , Christian Michelet (5) , Danièle Bentué-Ferrer (1)

1 Service de Pharmacologie [Rennes]
2 Fonction, structure et inactivation d'ARN bactériens
3 Service des maladies infectieuses et réanimation médicale [Rennes] = Infectious Disease and Intensive Care [Rennes]
4 CIC - Centre d'Investigation Clinique [Rennes]
5 Irset - Institut de recherche en santé, environnement et travail

A rapid and specific high-performance liquid chromatography method with UV detection (HPLC-UV) for the simultaneous determination of 12 beta-lactam antibiotics (amoxicillin, cefepime, cefotaxime, ceftazidime, ceftriaxone, cloxacillin, imipenem, meropenem, oxacillin, penicillin G, piperacillin, and ticarcillin) in small samples of human plasma is described. Extraction consisted of protein precipitation by acetonitrile. An Atlantis T3 analytical column with a linear gradient of acetonitrile and a pH 2 phosphoric acid solution was used for separation. Wavelength photodiode array detection was set either at 210 nm, 230 nm, or 298 nm according to the compound. This method is accurate and reproducible (coefficient of variation [CV] < 8%), allowing quantification of beta-lactam plasma levels from 5 to 250 μg/ml without interference with other common drugs. This technique is easy to use in routine therapeutic drug monitoring of beta-lactam antibiotics.

Domaines: Microbiologie et Parasitologie

Marie-Clémence Verdier, Olivier Tribut, Pierre Tattevin, Yves Le Tulzo, Christian Michelet, et al.. Simultaneous determination of 12 beta-lactam antibiotics in human plasma by high-performance liquid chromatography with UV detection: application to therapeutic drug monitoring.. Antimicrobial Agents and Chemotherapy, 2011, 55 (10), pp.4873-9. ⟨10.1128/AAC.00533-11⟩. ⟨hal-00875271⟩