Thyroid-stimulating hormone and free thyroxine on the ADVIA Centaur immunoassay system: A multicenter assessment of analytical performance.

Nathalie Reix (1, 2) , Catherine Massart (1, 3, 4) , Michèle d'Herbomez (1, 5) , Françoise Gasser (1, 2) , Béatrice Heurtault (2, 6) , Arnaud Agin (1, 2)

1 Société Française de Médecine Nucléaire
2 Laboratoire d'Hormonologie
3 Unité Fonctionnelle d'Hormonologie
4 CIC - Centre d'Investigation Clinique [Rennes]
5 Laboratoire de médecine nucléaire
6 CAMB - Conception et application de molécules bioactives

Objectives: We assessed the analytical performance of the TSH and FT4 assays on ADVIA Centaur in a multicenter national evaluation. Design and methods: A precision study and a method comparison were performed. Reference values stated by the manufacturer were checked from 379 normal subjects. Results: For TSH and FT4, the intra-assay CVs were below 2.3 and 5.2%, respectively, and the inter-assay CVs below 4.4% and 7.2%, respectively. Therefore, the precision and reproducibility were acceptable. Bland-Altman bias plots revealed good correlation and agreement with Cobas assays. TSH and FT4 data yielded reference ranges of 0.64-3.24 mIU/L and 10.5-18.9 pmol/L, respectively. Conclusion: These assays demonstrate reliable characteristics. The reference ranges obtained can be used for interpretation of thyroid function.

Mots clés: Thyroid-stimulating hormone; Free thyroxine; Evaluation; Reference values

Domaines: Médecine nucléaire

Nathalie Reix, Catherine Massart, Michèle d'Herbomez, Françoise Gasser, Béatrice Heurtault, et al.. Thyroid-stimulating hormone and free thyroxine on the ADVIA Centaur immunoassay system: A multicenter assessment of analytical performance.. Clinical Biochemistry, 2013, 46 (13-14), pp.1305-1308. ⟨10.1016/j.clinbiochem.2013.04.015⟩. ⟨hal-00916429⟩