Skip to Main content Skip to Navigation
Journal articles

BMP7 Expression Correlates with Secondary Drug Resistance in Mantle Cell Lymphoma

Abstract : Purpose We designed a gene profiling experiment to identify genes involved in secondary drug resistance in mantle cell lymphomas (MCL). Experimental Design We obtained paired tissue samples collected from the same patients before treatment and after relapse or progression. Variations in gene expression between the 2 samples were estimated for 5 patients. For each gene, the mean variation was estimated for patients with a refractory primary tumor and for responders who developed secondary drug resistance. Nine genes of interest were selected on the basis of the magnitude and statistical significance of the variation of expression in responders and non-responders. Results BMP7 was the only one with significantly increased expression at relapse in patients who developed secondary resistance. Validation of BMP7 as a key gene involved in secondary resistance was performed using cultures of cell line. Incubation of BMP7 with MCL cell lines increased their resistance to bortezomib and cytarabine, while inhibition of BMP7 expression by siRNA correlated with increased cell death linked to drug application. Conclusion Variations in gene expression after treatment point out BMP7 as a key gene involved in secondary resistance in mantle cell lymphoma.
Document type :
Journal articles
Complete list of metadatas

Cited literature [24 references]  Display  Hide  Download

https://hal-univ-rennes1.archives-ouvertes.fr/hal-00935237
Contributor : Morgane Le Corre <>
Submitted on : Thursday, January 23, 2014 - 11:41:12 AM
Last modification on : Wednesday, October 14, 2020 - 3:58:56 AM
Long-term archiving on: : Thursday, April 24, 2014 - 1:40:10 AM

File

BMP7_Expression_Correlates-ACC...
Publisher files allowed on an open archive

Identifiers

Citation

Valérie Camara-Clayette, Serge Koscielny, Sébastien Roux, Thierry Lamy, Jacques Bosq, et al.. BMP7 Expression Correlates with Secondary Drug Resistance in Mantle Cell Lymphoma. PLoS ONE, Public Library of Science, 2013, 8 (9), pp.e73993. ⟨10.1371/journal.pone.0073993⟩. ⟨hal-00935237⟩

Share

Metrics

Record views

443

Files downloads

335