Detecting Epigenetic Effects of Transposable Elements in Plants

Christian Parisod (1) , Armel Salmon (2) , Malika L. Aïnouche (2) , Marie-Angèle Grandbastien (1)

1 IJPB - Institut Jean-Pierre Bourgin
2 ECOBIO - Ecosystèmes, biodiversité, évolution [Rennes]

Transposable elements (TE) represent a major fraction of eukaryotic genomes and play many roles in plant epigenetics. In this chapter, we describe the use of Sequence-Specifi c Amplifi ed Polymorphism (SSAP) as a reliable Transposon Display technique applicable for use in many plant species. We also discuss the interpretation of SSAP data and associated risks. This technique has potential to allow rapid screening of plant populations, especially in nonmodel or wild species.

Keywords: Transposable element; Epigenetics; SSAP; Transposition

Domains: Life Sciences [q-bio]; Biodiversity; Systematics, Phylogenetics and taxonomy; Life Sciences [q-bio]; Genetics; Plants genetics

Christian Parisod, Armel Salmon, Malika L. Aïnouche, Marie-Angèle Grandbastien. Detecting Epigenetic Effects of Transposable Elements in Plants. Charles Spillane and Peter C. McKeown. Plant Epigenetics and Epigenomics: Methods and Protocols, 1112, Springer Science, pp.211-217, 2014, Methods in Molecular Biology, vol. 1112, 978-1-62703-772-3; 978-1-62703-773-0. ⟨10.1007/978-1-62703-773-0_14⟩. ⟨hal-00990560⟩