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The chronic lymphocytic leukemia clone disrupts the bone marrow microenvironment.

Abstract : The systematic localization of chronic lymphocytic leukemia (CLL) B-cells in the bone marrow (BM), together with the ex vivo protective effect of stromal cells on their spontaneous apoptosis, both indicate a specific role of the BM microenvironment. In vivo, the impact of CLL cells on mesenchymal stromal cells (MSC) remains a source of debate. Here, we quantified and expanded colony forming unit-fibroblasts (CFU-F) from CLL-BM under standard conditions, analyzed the expression of selected genes and studied secretion profiles. We observed failing of CLL-BM cultures in standard conditions (45.5% vs. <0.1%), and even after adding bFGF, there were fewer CFU-F than from normal bone marrow (NBM) (1.3 vs 40/106 cells respectively; p<0.01). Furthermore, their polygonal aspect and low proliferative capacity, together with the expression of 384 selected genes and a secreted set of molecules related to senescence-associated secretory phenotype (SASP) indicated a state of senescence, further confirmed by the higher proportion of senescence-associated ß-galactosidase (SA-βGAL) positive cells and p16INK4a overexpression. In our hands, hypoxic conditions (5% O2) did not rescue CFU-Fs. Given the role of MSC in BM tissue organization, we studied hematons which are generally considered to be elementary BM units. These structures were rare or had even disappeared completely. When hematons were present, we systematically observed nodular B-CLL cell invasion only. These data confirm that the B-CLL clone has a marked impact on MSC and disrupts BM organization in vivo, raising new questions about in vivo pathophysiology.
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https://hal-univ-rennes1.archives-ouvertes.fr/hal-01058950
Contributor : Laurent Jonchère <>
Submitted on : Thursday, August 28, 2014 - 4:47:54 PM
Last modification on : Thursday, February 7, 2019 - 5:30:30 PM

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Alexandre Janel, Frédérique Dubois-Galopin, Céline Bourgne, Juliette Berger, Karin Tarte, et al.. The chronic lymphocytic leukemia clone disrupts the bone marrow microenvironment.. Stem Cells and Development, Mary Ann Liebert, 2014, 23 (24), pp.2972-2982. ⟨10.1089/scd.2014.0229⟩. ⟨hal-01058950⟩

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