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Effects of a ceramic biomaterial on immune modulatory properties and differentiation potential of human mesenchymal stromal cells of different origin

Abstract : The aim of this study was to assess the immune modulatory properties of human mesenchymal stromal cells obtained from bone marrow (BM-MSCs), fat (ASCs) and cord blood (CB-MSCs) in presence of a hydroxyapatite and tricalcium-phosphate (HA/TCP) biomaterial as a scaffold for MSC delivery. In resting conditions, a short-term culture with HA/TCP did not modulate the anti-apoptotic and suppressive features of the various MSC types towards T, B and NK cells; in addition, when primed with inflammatory cytokines, MSCs similarly increased their suppressive capacities in presence or absence of HA/TCP. The long-term culture of BM-MSCs with HA/TCP induced an osteoblast-like phenotype with up-regulation of OSTERIX and OSTEOCALCIN, similarly to what obtained with dexamethasone and, to a higher extent, with BMP-4 treatment. MSC-derived osteoblasts did not trigger immune cell activation, but were less efficient than undifferentiated MSCs in inhibiting stimulated T and NK cells. Interestingly, their suppressive machinery included not only the activation of indoleamine-2,3 dioxygenase (IDO), which plays a central role in T cell inhibition, but also cyclooxygenase 2 (COX-2) that was not significantly involved in immune modulatory effect of human undifferentiated MSCs. As COX-2 is significantly involved in bone healing, its induction by HA/TCP could also contribute to the therapeutic activity of MSCs for bone tissue engineering.
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https://hal-univ-rennes1.archives-ouvertes.fr/hal-01076081
Contributor : Laurent Jonchère <>
Submitted on : Tuesday, October 21, 2014 - 9:57:41 AM
Last modification on : Monday, March 23, 2020 - 11:46:16 AM

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Giulio Bassi, Fabien Guilloton, Cedric Menard, Mariano Di Trapani, Frederic Deschaseaux, et al.. Effects of a ceramic biomaterial on immune modulatory properties and differentiation potential of human mesenchymal stromal cells of different origin. Tissue Engineering: Parts A, B, and C, Mary Ann Liebert, 2015, 21 (3-4), pp.767-781. ⟨10.1089/ten.TEA.2014.0269⟩. ⟨hal-01076081⟩

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