Molecular evolution and transcriptional regulation of the oilseed rape proline dehydrogenase genes suggest distinct roles of proline catabolism during development

Pascal Faës 1 Carole Deleu 1 Abdelkader Aïnouche 2 Françoise Le Cahérec 1 Emilie Montes 1 Vanessa Clouet 1 Anne-Marie Gouraud 1 Benjamin Albert 1 Mathilde Orsel 1 Gilles Lassalle 1 Laurent Leport 1 Alain Bouchereau 3 Marie-Françoise Niogret 1, *
* Corresponding author
1 IGEPP - Institut de Génétique, Environnement et Protection des Plantes
2 ECOBIO - Ecosystèmes, biodiversité, évolution [Rennes]
3 Rendement sous Contrainte Abiotique (RCA)
IGEPP - Institut de Génétique, Environnement et Protection des Plantes
Abstract : Proline dehydrogenase (ProDH) catalyzes the first step in the catabolism of proline. The ProDH gene family in oilseed rape (Brassica napus) was characterized and compared to other Brassicaceae ProDH sequences to establish the phylogenetic relationships between genes. Six Bna-ProDH1 genes and two BnaProDH2 genes were identified in the B. napus genome. Expression of the three paralogouspairs of BnaProDH1 genes and the two homoeologous BnaProDH2 genes was measured by real-time quantitative RT-PCR in plants at vegetative and reproductive stages. The BnaProDH2 genes are specifically expressed in vasculature in an age-dependent manner, while BnaProDH1 genes are strongly expressed in pollen grains and roots. Compared to the abundant expression of BnaProDH1, the overallexpression of BnaProDH2 is low except in roots and senescent leaves. The BnaProDH1 paralogs showed different levels of expression with BnaA&C.ProDH1.a the most strongly expressed and BnaA&C.ProDH1.c the least. The promoters of two BnaProDH1 and two BnaProDH2 genes were fused with uidA reporter gene (GUS) to characterize organ and tissue expression profiles in transformed B. napusplants. The transformants with promoters from different genes showed contrasting patterns of GUS activity, which corresponded to the spatial expression of their respective transcripts. ProDHs probably have non-redundant functions in different organs and at different phenological stages. In terms of molecular evolution, all BnaProDH sequences appear to have undergone strong purifying selection and some copies are becoming subfunctionalized. This detailed description of oilseed rape ProDH genes provides new elements to investigate the function of proline metabolism in plant development.
Keywords : Brassica napus L Leaf senescence Amino acid catabolism Subfunctionalization Polyploidy ProDH
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Journal articles
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https://hal-univ-rennes1.archives-ouvertes.fr/hal-01088770
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Submitted on : Friday, November 28, 2014 - 4:07:26 PM
Last modification on : Thursday, February 20, 2020 - 7:23:27 PM

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Pascal Faës, Carole Deleu, Abdelkader Aïnouche, Françoise Le Cahérec, Emilie Montes, et al.. Molecular evolution and transcriptional regulation of the oilseed rape proline dehydrogenase genes suggest distinct roles of proline catabolism during development. Planta, Springer Verlag, 2015, 241 (2), pp.403-419. ⟨10.1007/s00425-014-2189-9⟩. ⟨hal-01088770⟩

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