Skip to Main content Skip to Navigation
Journal articles

Self-Restrained B Cells Arise following Membrane IgE Expression

Abstract : Among immunoglobulins (Igs), IgE can powerfully contribute to antimicrobial immunity and severe allergy despite its low abundance. IgE protein and gene structure resemble other Ig classes, making it unclear what constrains its production to thousand-fold lower levels. Whether class-switched B cell receptors (BCRs) differentially control B cell fate is debated, and study of the membrane (m)IgE class is hampered by its elusive in vivo expression. Here, we demonstrate a self-controlled mIgE B cell stage. Primary or transfected mIgE cells relocate the BCRs into spontaneously internalized lipid rafts, lose mobility to chemokines, and change morphology. We suggest that combined proapoptotic mechanisms possibly involving Hax1 prevent mIgE memory lymphocyte accumulation. By uncoupling in vivo IgE switching from cytokine and antigen stimuli, we show that these features are independent from B cell stimulation and instead result from mIgE expression per se. Consequently, few cells survive IgE class switching, which might ensure minimal long-term IgE memory upon differentiation into plasma cells.
Document type :
Journal articles
Complete list of metadatas

Cited literature [42 references]  Display  Hide  Download

https://hal-univ-rennes1.archives-ouvertes.fr/hal-01116949
Contributor : Laurent Jonchère <>
Submitted on : Monday, February 16, 2015 - 9:47:02 AM
Last modification on : Monday, May 18, 2020 - 11:00:35 AM

File

Self-Restrained B Cells.pdf
Publisher files allowed on an open archive

Identifiers

Citation

Brice Laffleur, Sophie Duchez, Karin Tarte, Nicolas Denis-Lagache, Sophie Péron, et al.. Self-Restrained B Cells Arise following Membrane IgE Expression. Cell Reports, Elsevier Inc, 2015, 10 (6), pp.900-909. ⟨10.1016/j.celrep.2015.01.023⟩. ⟨hal-01116949⟩

Share

Metrics

Record views

417

Files downloads

576