An investigation of the endocrine-disruptive effects of bisphenol A in human and rat fetal testes.

Millissia Ben Maamar (1) , Laurianne Lesné (1) , Christèle Desdoits-Lethimonier (1) , Isabelle Coiffec (1) , Julie Lassurguère (1) , Vincent Lavoué (2) , Yoann Deceuninck (3) , Jean-Philippe Antignac (3) , Bruno Le Bizec (3) , Elisabeth Perdu (4) , Daniel Zalko (5) , Charles Pineau (1) , Cécile Chevrier (1) , Nathalie Dejucq-Rainsford (1) , Séverine Mazaud-Guittot (1) , Bernard Jégou (1, 6, 7)

1 Irset - Institut de recherche en santé, environnement et travail
2 Service de Gynécologie et Obstétrique [Rennes] = Gynaecology [Rennes]
3 LABERCA - Laboratoire d'étude des Résidus et Contaminants dans les Aliments
4 ToxAlim - ToxAlim
5 ToxAlim-MeX - Métabolisme et Xénobiotiques
6 EHESP - École des Hautes Études en Santé Publique [EHESP]
7 DSETGS - Département Santé Environnement Travail et Génie Sanitaire

Few studies have been undertaken to assess the possible effects of bisphenol A (BPA) on the reproductive hormone balance in animals or humans with often contradictory results. We investigated possible direct endocrine disruption by BPA of the fetal testes of 2 rat strains (14.5–17.5 days post-coitum) and humans (8–12 gestational weeks) and under different culture conditions. BPA concentrations of 10-8M and 10-5M for 72h reduced testosterone production by the Sprague-Dawley fetal rat testes, while only 10-5M suppressed it in the Wistar strain. The suppressive effects at 10-5M were seen as early as 24h and 48h in both strains. BPA at 10-7-10-5M for 72h suppressed the levels of fetal rat Leydig cell insulin-like factor 3 (INSL3). BPA exposure at 10-8M, 10-7M, and 10-5M for 72h inhibited testosterone production in fetal human testes. For the lowest doses, the effects observed occurred only when no gonadotrophin was added to the culture media and were associated with a poorly preserved testicular morphology. We concluded that (i) BPA can display anti-androgenic effects both in rat and human fetal testes; (ii) it is essential to ascertain that the divergent effects of endocrine disruptors between species in vitro do not result from the culture conditions used, and/or the rodent strain selected; (iii) the optimization of each in vitro assay for a given species should be a major objective rather than the search of an hypothetical trans-species consensual model-system, as the organization of the testis is intrinsically different between mammalian species; (iv) due to the uncertainty existing on the internal exposure of the human fetal testis to BPA, and the insufficient number of epidemiological studies on the endocrine disruptive effects of BPA, caution should be taken in the extrapolation of our present results to the human reproductive health after fetal exposure to BPA.

Keywords: Pollutants; Rats; Production control; Testes; Testosterone; Fetuses; Ethanol; Sertoli cells

Domains: Life Sciences [q-bio]; Toxicology; Life Sciences [q-bio]; Ecology, environment; Health

Millissia Ben Maamar, Laurianne Lesné, Christèle Desdoits-Lethimonier, Isabelle Coiffec, Julie Lassurguère, et al.. An investigation of the endocrine-disruptive effects of bisphenol A in human and rat fetal testes.. PLoS ONE, 2015, 10 (2), pp.e0117226. ⟨10.1371/journal.pone.0117226⟩. ⟨hal-01121759⟩