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Identification and frequency of circulating CD4(+) T lymphocytes specific to Benzylpenicillin in healthy donors.

Abstract : Drug hypersensitivity is known to rely on a drug-specific T-cell response. Amplitude of antigen-specific T-cell response is partly controlled by the size of the antigen-specific naïve CD4(+) T-cell repertoire, but estimate of this repertoire has never been investigated for allergenic drugs. The purpose of this study was to evaluate the frequency of benzylpenicillin-specific CD4(+) T lymphocytes in healthy donors. Co-cultures were established with CD4(+) T lymphocytes from healthy donors and mature autologous dendritic cells loaded with benzylpenicillin coupled to human serum albumin. CD4(+) T lymphocytes were stimulated once a week for 4 weeks with benzylpenicillin coupled to human serum albumin. The CD4(+) T-cell response was measured using an interferon-γ ELISPOT assay. Frequency of benzylpenicillin-specific naive CD4(+) T lymphocytes was then calculated using the Poisson distribution law. Results showed the presence of benzylpenicillin-specific CD4(+) T lymphocytes in 9 of 10 tested healthy donors irrespective of their HLA typing, with a mean frequency of 0.29 cells per million of CD4(+) T cells. Experiments performed on naive (CD45RA(+) ) and on memory (CD45RO(+) ) CD4(+) T lymphocytes showed that these benzylpenicillin-specific CD4(+) T lymphocytes belonged to the naive T-cell subpopulation. This study showed for the first time the existence of naive CD4(+) T lymphocytes specific to benzylpenicillin in healthy donors.
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https://hal-univ-rennes1.archives-ouvertes.fr/hal-01122172
Contributor : Laurent Jonchère <>
Submitted on : Tuesday, March 3, 2015 - 1:50:11 PM
Last modification on : Wednesday, September 16, 2020 - 5:05:15 PM

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C Nhim, S Delluc, F Halgand, L de Chaisemartin, R J Weaver, et al.. Identification and frequency of circulating CD4(+) T lymphocytes specific to Benzylpenicillin in healthy donors.. Allergy, Wiley, 2013, 68 (7), pp.899-905. ⟨10.1111/all.12173⟩. ⟨hal-01122172⟩

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