Skip to Main content Skip to Navigation
Journal articles

Effect of Diffusion on Discoloration of Congo Red by Alginate Entrapped Turnip (Brassica rapa) Peroxidase.

Abstract : Enzymatic discoloration of the diazo dye, Congo red (CR), by immobilized plant peroxidase from turnip "Brassica rapa" is investigated. Partially purified turnip peroxidase (TP) was immobilized by entrapment in spherical particles of calcium alginate and was assayed for the discoloration of aqueous CR solution. Experimental data revealed that pH, reaction time, temperature, colorant, and H2O2 concentration play a significant role in dye degradation. Maximum CR removal was found at pH 2.0, constant temperature of 40°C in the presence of 10 mM H2O2, and 180 mg/L of CR. More than 94% of CR was removed by alginate immobilized TP after 1 h of incubation in a batch process under optimal conditions. About 74% removal efficiency was retained after four recycles. Diffusional limitations in alginate beads such as effectiveness factor η, Thiele modulus Φ, and effective diffusion coefficients (D e ) of Congo red were predicted assuming a first-order biodegradation kinetic. Results showed that intraparticle diffusion resistance has a significant effect on the CR biodegradation rate.
Document type :
Journal articles
Complete list of metadatas

Cited literature [50 references]  Display  Hide  Download

https://hal-univ-rennes1.archives-ouvertes.fr/hal-01134162
Contributor : Laurent Jonchère <>
Submitted on : Monday, March 23, 2015 - 9:40:07 AM
Last modification on : Friday, July 10, 2020 - 4:26:37 PM
Long-term archiving on: : Thursday, July 2, 2015 - 6:21:00 AM

File

575618.pdf
Publisher files allowed on an open archive

Identifiers

Citation

Afaf Ahmedi, Mahmoud Abouseoud, Abdeltif Amrane, Annabelle Couvert. Effect of Diffusion on Discoloration of Congo Red by Alginate Entrapped Turnip (Brassica rapa) Peroxidase.. Enzyme Research, Hindawi Publishing Corporation, 2015, 2015, pp.575618. ⟨10.1155/2015/575618⟩. ⟨hal-01134162⟩

Share

Metrics

Record views

303

Files downloads

386