Skip to Main content Skip to Navigation
New interface
Journal articles

TAT and HA2 Facilitate Cellular Uptake of Gold Nanoparticles but Do Not Lead to Cytosolic Localisation.

Abstract : The methods currently available to deliver functional labels and drugs to the cell cytosol are inefficient and this constitutes a major obstacle to cell biology (delivery of sensors and imaging probes) and therapy (drug access to the cell internal machinery). As cell membranes are impermeable to most molecular cargos, viral peptides have been used to bolster their internalisation through endocytosis and help their release to the cytosol by bursting the endosomal vesicles. However, conflicting results have been reported on the extent of the cytosolic delivery achieved. To evaluate their potential, we used gold nanoparticles as model cargos and systematically assessed how the functionalisation of their surface by either or both of the viral peptides TAT and HA2 influenced their intracellular delivery. We evaluated the number of gold nanoparticles present in cells after internalisation using photo-thermal microscopy and their subcellular localisation by electron microscopy. While their uptake increased when the TAT and/or HA2 viral peptides were present on their surface, we did not observe a significant cytosolic delivery of the gold nanoparticles.
Document type :
Journal articles
Complete list of metadata

Cited literature [74 references]  Display  Hide  Download
Contributor : Laurent Jonchère Connect in order to contact the contributor
Submitted on : Tuesday, April 7, 2015 - 9:49:13 AM
Last modification on : Wednesday, March 30, 2022 - 2:34:23 PM


TAT and HA2 Facilitate Cellula...
Publisher files allowed on an open archive



Yann Cesbron, Umbreen Shaheen, Paul Free, Raphaël Lévy. TAT and HA2 Facilitate Cellular Uptake of Gold Nanoparticles but Do Not Lead to Cytosolic Localisation.. PLoS ONE, 2015, 10 (4), pp.e0121683. ⟨10.1371/journal.pone.0121683.s006⟩. ⟨hal-01139794⟩



Record views


Files downloads