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Control of E-cadherin apical localisation and morphogenesis by a SOAP-1/AP-1/clathrin pathway in C. elegans epidermal cells.

Abstract : E-cadherin (E-cad) is the main component of epithelial junctions in multicellular organisms, where it is essential for cell-cell adhesion. The localisation of E-cad is often strongly polarised in the apico-basal axis. However, the mechanisms required for its polarised distribution are still largely unknown. We performed a systematic RNAi screen in vivo to identify genes required for the strict E-cad apical localisation in C. elegans epithelial epidermal cells. We found that the loss of clathrin, its adaptor AP-1 and the AP-1 interactor SOAP-1 induced a basolateral localisation of E-cad without affecting the apico-basal diffusion barrier. We further found that SOAP-1 controls AP-1 localisation, and that AP-1 is required for clathrin recruitment. Finally, we also show that AP-1 controls E-cad apical delivery and actin organisation during embryonic elongation, the final morphogenetic step of embryogenesis. We therefore propose that a molecular pathway, containing SOAP-1, AP-1 and clathrin, controls the apical delivery of E-cad and morphogenesis.
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https://hal-univ-rennes1.archives-ouvertes.fr/hal-01141743
Contributor : Laurent Jonchère <>
Submitted on : Monday, April 13, 2015 - 4:14:49 PM
Last modification on : Monday, March 2, 2020 - 9:51:19 AM

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Ghislain Gillard, Massiullah Shafaq-Zadah, Ophélie Nicolle, Raghida Damaj, Jacques Pécréaux, et al.. Control of E-cadherin apical localisation and morphogenesis by a SOAP-1/AP-1/clathrin pathway in C. elegans epidermal cells.. Development (Cambridge, England), Company of Biologists, 2015, 142 (9), pp.1684-1694. ⟨10.1242/dev.118216⟩. ⟨hal-01141743⟩

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