Diversity and comparative genomics of Microviridae in Sphagnum-dominated peatlands

Abstract : Microviridae, a family of bacteria-infecting ssDNA viruses, is one of the still poorly characterized bacteriophage groups, event hough it includes phage PhiX174, one of the main models in virology for genomic and capsid structure studies. Recent studies suggest that they are diverse and well represented in marine and freshwater virioplankton as well as in human microbiomes. However,theirdiversity,abundance,andecologicalrolearecompletelyunknowninsoilecosystems.Herewepresentthecomparativeanalysisof17completelyassembledMicroviridaegenomesfrom12viromesofaSphagnum-dominatedpeatland.PhylogeneticanalysisoftheconservedmajorcapsidproteinsequencesrevealedtheaffiliationtoGokushovirinaeandPichovirinaeaswellastotwonewlydefinedsubfamilies,theAravirinaeandStokavirinae.Additionally,twonewdistinctprophageswereidentifiedinthegenomesofParabacteroidesmerdaeandParabacteroidesdistasonisrepresentingapotentialnewsubfamilyofMicroviridae.Thedifferentiationofthesubfamilieswasconfirmedbygeneorderandsimilarityanalysis.Relativeabundanceanalysisusingtheaffiliationofthemajorcapsidprotein(VP1)revealedthatGokushovirinae,followedbyAravirinae,arethemostabundantMicroviridaein11outof12peatviromes.SequencesmatchingtheGokushovirinaeandAravirinaeVP1matchingsequences,respectively,accountedforupto4.19and0.65%ofthetotalnumberofsequencesinthecorrespondingvirome,respectively.InthisstudyweprovidenewgenomeinformationofMicroviridaeandpavethewaytowardquantitativeestimationsofMicroviridaesubfamilies.
Document type :
Journal articles
Complete list of metadatas

https://hal-univ-rennes1.archives-ouvertes.fr/hal-01149546
Contributor : Valerie Briand <>
Submitted on : Thursday, May 7, 2015 - 11:39:05 AM
Last modification on : Friday, May 24, 2019 - 4:59:49 PM

Links full text

Identifiers

Citation

Achim Quaiser, Alexis Dufresne, Flore Ballaud, Simon Roux, Yvan Zivanovic, et al.. Diversity and comparative genomics of Microviridae in Sphagnum-dominated peatlands. Frontiers in microbiology, Frontiers Research Foundation, 2015, 6, pp.375. ⟨10.3389/fmicb.2015.00375⟩. ⟨hal-01149546⟩

Share

Metrics

Record views

442