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Streptozotocin induced diabetes alone or in combination with exercise does not affect rcan 1 in rat skeletal muscles.

Abstract : Regulator of Calcineurin1 (RCAN1) is a calcineurin modulator protein. Calcineurin is ubiquitously expressed Sr/Thr phosphatase under the control of calcium/calmodulin which has been identified in skeletal muscles. Thus far, RCAN1, being an oxidative and high-glucose responsive element, has not been studied under conditions of diabetes and physical exercise. Experiment: 16male Wistar rats received intraperitoneal streptozotocin injection while 16 other were kept as control. Once diabetic state was confirmed, each group was divided into two sub-groups of trained and non-trained. The training consisted of an 8 weeks of treadmill exercise (10° incline, up to 25 mmin-1, 60min/day, 70% VO2). Three different skeletal muscles were collected: Soleus, a predominantly slow twitch muscle rich in type 1 fibers; Extensor Digitorum Longus (EDL), a fast twitch muscle rich in type 2 fibers; and Gastrocnemius a ‘mixed muscle’ which contains both type 1 and 2 fibers. Results: Muscle atrophy and hyperglycemia were confirmed in diabetic rats. Calcineurin, PGC1-alpha and three main RCAN1 isoforms namely; RCAN 1-1L, RCAN1-1S and RCAN1-4 were detected in the three muscles. Neither of them has been modified following the treatments. Diabetes did not modified protein carbonyls in the muscles and TBARS augmented only in soleus after diabetes induction. Conclusion: The streptozotocin induced diabetes and the exercise protocol were not strong enough to bring about an oxidative stress in the muscles, therefore RCAN1 expression was not altered. Our findings are consistent with the previews studies, showing that stress can modulate RCAN1 expression and in the absence of stress RCAN1 remains unchanged.
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Conference papers
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https://hal-univ-rennes1.archives-ouvertes.fr/hal-01159330
Contributor : Laurent Jonchère <>
Submitted on : Wednesday, June 3, 2015 - 11:11:07 AM
Last modification on : Wednesday, October 14, 2020 - 4:03:36 AM

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  • HAL Id : hal-01159330, version 1

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Ramin Emrani Bidi, Amélie Rébillard, Dany Saligaut, Luz Lefeuvre-Orfila, Arlette Gratas-Delamarche, et al.. Streptozotocin induced diabetes alone or in combination with exercise does not affect rcan 1 in rat skeletal muscles.. Colloque BIOMED 7-8 mai 2015 Québec, Canada, 2015, Laurentides, Canada. ⟨hal-01159330⟩

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