Phenolic composition, in vitro antioxidant effects and tyrosinase inhibitory activity of three Algerian Mentha species: M. spicata (L.), M. pulegium (L.) and M. rotundifolia (L.) Huds (Lamiaceae)

Fatiha Brahmi; Didier Hauchard; Naima Guendouze; Khodir Madani; Martin Kiendrebeogo; Léocadie Kamagaju; Caroline Stévigny; Mohamed Chibane; Pierre Duez

Phenolic composition, in vitro antioxidant effects and tyrosinase inhibitory activity of three Algerian Mentha species: M. spicata (L.), M. pulegium (L.) and M. rotundifolia (L.) Huds (Lamiaceae)

Fatiha Brahmi ¹ Didier Hauchard ² Naima Guendouze ¹ Khodir Madani ¹ Martin Kiendrebeogo ³ Léocadie Kamagaju ⁴ Caroline Stévigny ⁴ Mohamed Chibane ⁵ Pierre Duez ^{4, 6}

1 Université Abderrahmane Mira [Béjaïa]

2 ISCR - Institut des Sciences Chimiques de Rennes

3 Lab Biochim & Chim Appl LABIOCA

4 ULB - Université Libre de Bruxelles [Bruxelles]

5 UMAOB - Université Mohamed Akli Ouelhadj de Bouira

6 UMons - Université de Mons

Abstract : The leaves of three Mentha species harvested in Algeria, Mentha spicata L. (MS), Mentha pulegium L. (MP) and Mentha rotundifolia (L.) Huds (MR) were examined for their content in polyphenols and for some activities-linked biological properties these could impart. The contents in total phenolics (TPC) and flavonoids (TFC) were evaluated by the Folin–Ciocalteu and the aluminum chloride methods, respectively. Whereas MS showed the highest TPC (12.0 ± 0.3 mg gallic acid equivalents/g of dry weight), MR had the highest content in TFC (3.3 ± 0.1 mg quercetin equivalents of dry weight). The pharmacological properties of these extracts were evaluated by assessing in vitro their antioxidant and antityrosinase activities. The modulation of mushroom tyrosinase activity was measured by colorimetry of the melanins formed in the presence of tyrosine. MS exhibited the strongest radical scavenging activity (RSA) in all assays: (i) the IC50s values to neutralize the 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) cation radicals (ABTS+) and the 2,2′-diphenyl-1-picrylhydrazyl radicals (DPPH) were 10.3 ± 0.9 and 16.2 ± 0.2 μg/mL, respectively; and (ii) its original electrochemically measured superoxide quenching index value is 188 ± 37 μg/mL (AI50). MR however showed the highest tyrosinase inhibitory activity (IC50 = 108 ± 20 μg/mL). A silica gel thin-layer chromatography (TLC) technique revealed the presence of caffeic and rosmarinic acids and diosmin in all extracts. These results were confirmed by high performance liquid chromatography with diode array detection (HPLC/DAD)

Keywords : Phenolic compounds Antioxidant activities TLC/HPLC Tyrosinase Mentha ssp.

Document type :

Journal articles

Domain :

Chemical Sciences

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Phenolic composition, in vitro antioxidant effects and tyrosinase inhibitory activity of three Algerian Mentha species: M. spicata (L.), M. pulegium (L.) and M. rotundifolia (L.) Huds (Lamiaceae)

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Phenolic composition, in vitro antioxidant effects and tyrosinase inhibitory activity of three Algerian Mentha species: M. spicata (L.), M. pulegium (L.) and M. rotundifolia (L.) Huds (Lamiaceae)

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