Porphyromonas gingivalis and Treponema denticola Exhibit Metabolic Symbioses

Kheng H. Tan Christine A. Seers Stuart G. Dashper Helen L. Mitchell James S. Pyke Vincent Meuric 1 Nada Slakeski Steven M. Cleal Jenny L. Chambers Malcolm J. Mcconville Eric C Reynolds
1 Microbiologie : Risques Infectieux
Abstract : Author SummaryUnlike the traditional view that most diseases are caused by infection with a single bacterial species, some chronic diseases including periodontitis result from the perturbation of the natural microbiota and the proliferation of a number of opportunistic pathogens. Both Porphyromonas gingivalis and Treponema denticola have been associated with the progression and severity of chronic periodontitis and have been shown to display synergistic virulence in animal models. However, the underlying mechanisms to these observations are unclear. Here we demonstrate that these two bacteria grow synergistically in continuous co-culture and modify their gene expression. The expression of T. denticola genes encoding known virulence factors and enzymes involved in the uptake and metabolism of the amino acid glycine was up-regulated in co-culture. T. denticola stimulated the proteolytic P. gingivalis to produce free glycine, which T. denticola used as a major carbon source. Our study shows P. gingivalis and T. denticola co-operate metabolically and this helps to explain their synergistic virulence in animal models and their intimate association in vivo
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Journal articles
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https://hal-univ-rennes1.archives-ouvertes.fr/hal-01188856
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Submitted on : Monday, August 31, 2015 - 4:17:53 PM
Last modification on : Wednesday, May 16, 2018 - 11:23:29 AM

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UNIV-RENNES1 | BIOSIT | NUMECAN | NUMECAN-CIMIAD | UR1-UFR-SVE | UNIV-RENNES

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Kheng H. Tan, Christine A. Seers, Stuart G. Dashper, Helen L. Mitchell, James S. Pyke, et al.. Porphyromonas gingivalis and Treponema denticola Exhibit Metabolic Symbioses. PLoS Pathogens, Public Library of Science, 2014, 10 (3), pp.e1003955. ⟨10.1371/journal.ppat.1003955⟩. ⟨hal-01188856⟩

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