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Journal articles
The formation of ordered nanoclusters controls cadherin anchoring to actin and cell-cell contact fluidity
Abstract : Oligomerization of cadherins could provide the stability to ensure tissue cohesion. Cadherins mediate cell-cell adhesion by forming trans-interactions. They form cis-interactions whose role could be essential to stabilize intercellular junctions by shifting cadherin clusters from a fluid to an ordered phase. However, no evidence has been provided so far for cadherin oligomerization in cellulo and for its impact on cell-cell contact stability. Visualizing single cadherins within cell membrane at a nanometric resolution, we show that E-cadherins arrange in ordered clusters, providing the first demonstration of the existence of oligomeric cadherins at cell-cell contacts. Studying the consequences of the disruption of the cis-interface, we show that it is not essential for adherens junction formation. Its disruption, however, increased the mobility of junctional E-cadherin. This destabilization strongly affected E-cadherin anchoring to actin and cell-cell rearrangement during collective cell migration, indicating that the formation of oligomeric clusters controls the anchoring of cadherin to actin and cell-cell contact fluidity.
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01196599
Contributor : Laurent Jonchère <>
Submitted on : Thursday, September 10, 2015 - 10:39:17 AM
Last modification on : Saturday, April 11, 2020 - 1:59:05 AM
Contributor : Laurent Jonchère <>
Submitted on : Thursday, September 10, 2015 - 10:39:17 AM
Last modification on : Saturday, April 11, 2020 - 1:59:05 AM
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