Proteins Involved in the Interaction of Potato Tubers with Pectobacterium atrosepticum: a Proteomic Approach to Understanding Partial Resistance

Marie-Renée Barzic (1) , Emmanuelle Com (2)

1 IGEPP - Institut de Génétique, Environnement et Protection des Plantes
2 Irset - Institut de recherche en santé, environnement et travail

Potato can be severely affected by various pathogens, including Pectobacterium atrosepticum, the cause of bacterial soft rot on tubers and of blackleg on stems. To date, no complete resistance to P.similar to atrosepticum is available, so that only cultivars exhibiting partial resistance can be found. The mechanistic basis of this type of resistance is still poorly understood. A proteomic approach was thus developed to identify pathways specifically activated during the interaction between potato tubers and P.similar to atrosepticum. Protein profiles on silver-stained gels in the 58 similar to pH range were obtained from healthy and infected tubers from two cultivars differing for resistance level and analyzed by 2-DE and nano-LC-MS/MS. Thirteen proteins were differentially up-regulated in the partially resistant cv. Kerpondy; by contrast, no significant differences in protein profiles of inoculated and control tubers were observed in the susceptible cv. Bintje. Mass spectrometry and database searching showed that these proteins are involved in energetic metabolism (glyceraldehyde-3-phosphate dehydrogenase, 2-phosphoglycerate dehydratase or enolase, fructose biphosphate aldolase and ATPase a subunit), cytoskeleton structure (actin), protein catabolism (cysteine protease inhibitor) and patatins or patatin precursors. Their involvement in defence responses of cv. Kerpondy to P.similar to atrosepticum is discussed. Proteomic appears as an efficient approach to have insight into the mechanisms and pathways leading to potato resistance against P.similar to atrosepticum.

Mots clés: DIFFERENCE GEL-ELECTROPHORESIS; PATHOGEN ERWINIA-CAROTOVORA; DEFENSE GENE-EXPRESSION; PLANT-PATHOGEN; PHYTOPHTHORA-INFESTANS; III EFFECTOR PROTEINS; SALICYLIC-ACID; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE; TOBACCO-LEAVES; CAROTOVORA SUBSP ATROSEPTICA; mass spectrometry; two-dimensional electrophoresis; tuber proteome; potato; Pectobacterium atrosepticum; partial resistance

Domaines: Sciences du Vivant [q-bio]

Marie-Renée Barzic, Emmanuelle Com. Proteins Involved in the Interaction of Potato Tubers with Pectobacterium atrosepticum: a Proteomic Approach to Understanding Partial Resistance. Journal of Phytopathology, 2012, 160 (10), pp.561 - 575. ⟨10.1111/j.1439-0434.2012.01950.x⟩. ⟨hal-01208654⟩