PAR-4 and anillin regulate myosin to coordinate spindle and furrow position during asymmetric division - Université de Rennes Accéder directement au contenu
Article Dans Une Revue Journal of Cell Biology Année : 2015

PAR-4 and anillin regulate myosin to coordinate spindle and furrow position during asymmetric division

Résumé

During asymmetric cell division, the mitotic spindle and polarized myosin can both determine the position of the cytokinetic furrow. However, how cells coordinate signals from the spindle and myosin to ensure that cleavage occurs through the spindle midzone is unknown. Here, we identify a novel pathway that is essential to inhibit myosin and coordinate furrow and spindle positions during asymmetric division. In Caenorhabditis elegans one-cell embryos, myosin localizes at the anterior cortex whereas the mitotic spindle localizes toward the posterior. We find that PAR-4/LKB1 impinges on myosin via two pathways, an anillin-dependent pathway that also responds to the cullin CUL-5 and an anillin-independent pathway involving the kinase PIG-1/MELK. In the absence of both PIG-1/MELK and the anillin ANI-1, myosin accumulates at the anterior cortex and induces a strong displacement of the furrow toward the anterior, which can lead to DNA segregation defects. Regulation of asymmetrically localized myosin is thus critical to ensure that furrow and spindle midzone positions coincide throughout cytokinesis.
Fichier principal
Vignette du fichier
PAR-4 and anillin regulate myosin.pdf (9.17 Mo) Télécharger le fichier
JCB_201503006_sm.pdf (1.95 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Origine : Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

hal-01214010 , version 1 (20-10-2015)

Licence

Paternité - Pas d'utilisation commerciale - Pas de modification

Identifiants

Citer

Anne Pacquelet, Perrine Uhart, Jean-Pierre Tassan, Grégoire Michaux. PAR-4 and anillin regulate myosin to coordinate spindle and furrow position during asymmetric division. Journal of Cell Biology, 2015, 210 (7), pp.1085--1099. ⟨10.1083/jcb.201503006⟩. ⟨hal-01214010⟩
123 Consultations
205 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More