Skip to Main content Skip to Navigation
Journal articles

Ptbp1 and Exosc9 knockdowns trigger skin stability defects through different pathways

Abstract : In humans, genetic diseases affecting skin integrity (genodermatoses) are generally caused by mutations in a small number of genes that encode structural components of the dermal-epidermal junctions. In this article, we first show that inactivation of both exosc9, which encodes a component of the RNA exosome, and ptbp1, which encodes an RNA-binding protein abundant in Xenopus embryonic skin, impairs embryonic Xenopus skin development, with the appearance of dorsal blisters along the anterior part of the fin. However, histological and electron microscopy analyses revealed that the two phenotypes are distinct. Exosc9 morphants are characterized by an increase in the apical surface of the goblet cells, loss of adhesion between the sensorial and peridermal layers, and a decrease in the number of ciliated cells within the blisters. Ptbp1 morphants are characterized by an altered goblet cell morphology. Gene expression profiling by deep RNA sequencing showed that the expression of epidermal and genodermatosis-related genes is also differentially affected in the two morphants, indicating that alterations in post-transcriptional regulations can lead to skin developmental defects through different routes. Therefore, the developing larval epidermis of Xenopus will prove to be a useful model for dissecting the post-transcriptional regulatory network involved in skin development and stability with significant implications for human diseases.
Document type :
Journal articles
Complete list of metadata

Cited literature [52 references]  Display  Hide  Download
Contributor : Laurent Jonchère Connect in order to contact the contributor
Submitted on : Monday, January 25, 2016 - 11:34:07 AM
Last modification on : Wednesday, March 30, 2022 - 2:37:06 PM
Long-term archiving on: : Tuesday, April 26, 2016 - 10:47:50 AM


Ptbp1 and Exosc9 knockdowns tr...
Files produced by the author(s)



Maud Noiret, Stéphanie Mottier, Gaelle Angrand, Carole Gautier-Courteille, Hubert Lerivray, et al.. Ptbp1 and Exosc9 knockdowns trigger skin stability defects through different pathways. Developmental Biology, Elsevier, 2016, 409 (2), pp.489-501. ⟨10.1016/j.ydbio.2015.11.002⟩. ⟨hal-01225487⟩



Record views


Files downloads