Combining two-dimensional gel electrophoresis and metabolomic data in support of dry-season survival in the two main species of the malarial mosquito Anopheles gambiae

Kévin Hidalgo (1, 2) , Karine Mouline (1, 3) , W. Mamai (3, 4, 1) , Natacha Foucreau (5) , Rock K. Dabiré (3) , Alain Bouchereau (6) , Frédéric Simard (7) , D Renault (2)

1 MIVEGEC - Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle
2 ECOBIO - Ecosystèmes, biodiversité, évolution [Rennes]
3 IRSS - Institut de Recherche en Sciences de la Santé
4 IRD - Institut de Recherche pour le Développement
5 LEHNA - Laboratoire d'Ecologie des Hydrosystèmes Naturels et Anthropisés
6 IGEPP - Institut de Génétique, Environnement et Protection des Plantes
7 MIVEGEC-DEEVA - Diversity, ecology, evolution & Adaptation of arthropod vectors

In dry savannahs of West-Africa, the malarial mosquitoes of the Anopheles gambiae sensu stricto complex annually survive the harsh desiccating conditions of the dry season. However, the physiological and biochemical mechanisms underlying how these mosquitoes survive such desiccating conditions are still undefined, and controversial. In this context, we provide the first work examining both proteomic and metabolomic changes in the two molecular forms of A. gambiae s.s (M and S forms) experimentally exposed to the rainy and dry season conditions as they experience in the field. Protein abundances of the mosquitoes were measured using a two-dimensional fluorescence difference gel electrophoresis (2D DIGE) coupled with a matrix-assisted laser desorption/ionisation-time of flight (MALDI-TOF) and tandem mass spectrometry (MS) for protein identification. These assays were conducted by Applied Biomics (http://www.appliedbiomics.com, Applied Biomics, Inc. Hayward, CA, USA), and the mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium (http://proteomecentral.proteomexchange.org) via the PRIDE partner repository with the dataset identifier PXD000294. The metabolomic analysis was conducted using both Acquity UPLC® system (for amino acid identification), and a gas-chromatography-mass spectrometry platform (for sugars identification). Metabolomic fingerprintings were assessed in the University of Rennes 1, UMR CNRS 6553 EcoBio (France). A detailed interpretation of the obtained data can be found in Hidalgo et al. (2014) [1] (Journal of Insect Physiology (2014)).

Keywords: Mosquito; Metabolomic; Amino acid; Sugar; Proteomic; 2D electrophoresis

Domains: Life Sciences [q-bio]; Animal biology; Invertebrate Zoology; Environmental Sciences; Biodiversity and Ecology

Kévin Hidalgo, Karine Mouline, W. Mamai, Natacha Foucreau, Rock K. Dabiré, et al.. Combining two-dimensional gel electrophoresis and metabolomic data in support of dry-season survival in the two main species of the malarial mosquito Anopheles gambiae. Data in Brief, 2015, 5, pp.255-268. ⟨10.1016/j.dib.2015.08.031⟩. ⟨hal-01234296⟩