Spindle assembly checkpoint inactivation fails to suppress neuroblast tumour formation in aurA mutant Drosophila

Renaud Caous (1) , Aude Pascal (1) , Pierre Romé (2) , Laurent Richard-Parpaillon (1) , Roger Karess (3) , Régis Giet (1)

1 IGDR - Institut de Génétique et Développement de Rennes
2 IGDR - Institut de Génétique et Développement de Rennes
3 IJM (UMR_7592) - Institut Jacques Monod

Tissue homeostasis requires accurate control of cell proliferation, differentiation and chromosome segregation. Drosophila sas-4 and aurA mutants present brain tumours with extra neuroblasts (NBs), defective mitotic spindle assembly and delayed mitosis due to activation of the spindle assembly checkpoint (SAC). Here we inactivate the SAC in aurA and sas-4 mutants to determine whether the generation of aneuploidy compromises NB proliferation. Inactivation of the SAC in the sas-4 mutant impairs NB proliferation and disrupts euploidy. By contrast, disrupting the SAC in the aurA mutant does not prevent NB amplification, tumour formation or chromosome segregation. The monitoring of Mad2 and cyclin B dynamics in live aurA NBs reveals that SAC satisfaction is not coupled to cyclin B degradation. Thus, the NBs of aurA mutants present delayed mitosis, with accurate chromosome segregation occurring in a SAC-independent manner. We report here the existence of an Aurora A-dependent mechanism promoting efficient, timed cyclin B degradation

Domaines: Sciences du Vivant [q-bio]

Renaud Caous, Aude Pascal, Pierre Romé, Laurent Richard-Parpaillon, Roger Karess, et al.. Spindle assembly checkpoint inactivation fails to suppress neuroblast tumour formation in aurA mutant Drosophila. Nature Communications, 2015, 6 (1), pp.8879. ⟨10.1038/ncomms9879⟩. ⟨hal-01237075⟩