Sample preparation for an optimized extraction of localized metabolites in lichens: Application to Pseudevernia furfuracea

Sarah Komaty (1) , Marine Letertre (1) , Huyen Duong Dang (1) , Harald Jungnickel (2) , Peter Laux (2) , Andreas Luch (2) , Daniel Carrié (1) , Odile Merdrignac-Conanec (1) , Jean-Pierre Bazureau (1) , Fabienne Gauffre (1) , Sophie Tomasi (1) , Ludovic Paquin (1)

1 ISCR - Institut des Sciences Chimiques de Rennes
2 BfR - Bundesinstitut für Risikobewertung - Federal Institute for Risk Assessment

Lichens are symbiotic organisms known for producing unique secondary metabolites with attractive cosmetic and pharmacological properties. In this paper, we investigated three standard methods of preparation of Pseudevernia furfuracea (blender grinding, ball milling, pestle and mortar). The materials obtained were characterized by electronic microscopy, nitrogen adsorption and compared from the point of view of extraction. Their microscopic structure is related to extraction efficiency. In addition, it is shown using thalline reactions and mass spectrometry mapping (TOF-SIMS) that these metabolites are not evenly distributed throughout the organism. Particularly, atranorin (a secondary metabolite of interest) is mainly present in the cortex of Pseudevernia furfuracea. Finally, using microwave assisted extraction (MAE) we obtained evidence that an appropriate preparation can increase the extraction efficiency of atranorin by a factor of five

Keywords: Secondary metabolite; Grinding process; Lichen; Tof-SIMS; Microwave extraction

Domains: Chemical Sciences

Sarah Komaty, Marine Letertre, Huyen Duong Dang, Harald Jungnickel, Peter Laux, et al.. Sample preparation for an optimized extraction of localized metabolites in lichens: Application to Pseudevernia furfuracea. Talanta, 2016, 150, pp.525-530. ⟨10.1016/j.talanta.2015.12.081⟩. ⟨hal-01254800⟩