Antioxidant supplementation accelerates cachexia development by promoting tumor growth in C26 tumor-bearing mice - Archive ouverte HAL Access content directly
Journal Articles Free Radical Biology and Medicine Year : 2016

Antioxidant supplementation accelerates cachexia development by promoting tumor growth in C26 tumor-bearing mice

(1) , (1) , (1) , (1)
1

Abstract

More than 50% of patients with advanced stages of colon cancer suffer from progressive loss of skeletal muscle, called cachexia, resulting in reduced quality of life and shortened survival. It is becoming evident that reactive oxygen species (ROS) regulate pathways controlling skeletal muscle atrophy. Herein we tested the hypothesis that antioxidant supplementation could prevent skeletal muscle atrophy in a model of cachectic Colon 26 (C26) tumor-bearing mice. Seven-week-old BALB/c mice were subcutaneously inoculated with colon 26 (C26) cancer cells or PBS. Then C26-mice were daily gavaged during 22 days either with PBS (vehicle) or an antioxidant cocktail whose composition is close to that of commercial dietary antioxidant supplements (rich in catechins, quercetin and vitamin C). We found that antioxidants enhanced weight loss and caused premature death of mice. Antioxidants supplementation failed to prevent (i) the increase in plasma TNF-α levels and systemic oxidative damage, (ii) skeletal muscle atrophy and (iii) activation of the ubiquitin-proteasome system (MuRF-1, MAFbx and polyubiquitinated proteins). Accordingly, immunohistological staining for Ki-67 and the expression of cell cycle inhibitors demonstrated that tumor of supplemented mice developed faster with a concomitant decrease in oxidative damage. Previous studies have shown that the use of catechins and quercetin separately can improve the musculoskeletal function in cachectic animals. However, our results indicate that the combination of these antioxidants reduced survival and enhanced cachexia in C26-mice.
Fichier principal
Vignette du fichier
Antioxidant supplementation accelerates cachexia_accepted.pdf (982.72 Ko) Télécharger le fichier
Origin : Files produced by the author(s)
Loading...

Dates and versions

hal-01255843 , version 1 (25-03-2016)

Identifiers

Cite

Mohamad Assi, Frédéric Derbré, Luz Lefeuvre-Orfila, Amélie Rébillard. Antioxidant supplementation accelerates cachexia development by promoting tumor growth in C26 tumor-bearing mice. Free Radical Biology and Medicine, 2016, 91, pp.204-214. ⟨10.1016/j.freeradbiomed.2015.12.019⟩. ⟨hal-01255843⟩
479 View
569 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More