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Stable-Isotope Probing RNA to Study Plant/Fungus Interactions

Abstract : The use of stable-isotope probing (SIP) allows tracing specific labeled substrates into fungi leading to a better understanding of their role in biogeochemical cycles and their relationship with their environment. Stable-isotope probing combined with ribosomal RNA molecule, conserved in the three kingdoms of life, and messenger RNA analysis permits the linkage of diversity and function. Here, we describe two methods designed to investigate the interactions between plant and its associated mycorrhizal compartment by tracing carbon flux from the host plant to its symbionts.
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https://hal-univ-rennes1.archives-ouvertes.fr/hal-01263106
Contributor : Laurent Jonchère <>
Submitted on : Wednesday, January 27, 2016 - 1:55:09 PM
Last modification on : Wednesday, February 26, 2020 - 9:09:00 AM

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Amandine Lê Van, Marie Duhamel, Achim Quaiser, Philippe Vandenkoornhuyse. Stable-Isotope Probing RNA to Study Plant/Fungus Interactions. Methods in Molecular Biology, Humana Press/Springer Imprint, 2016, 1399, pp.151--166. ⟨10.1007/978-1-4939-3369-3_9⟩. ⟨hal-01263106⟩

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