Skip to Main content Skip to Navigation
Journal articles

Single Wavelength Excitation Dual Color Flim for Multiplexing Genetically Encoded FRET Biosensors

Abstract : 2555-Plat. Genetically encoded Förster Resonance Energy Transfert (FRET) biosensors are powerful tools for monitoring spatiotemporal biochemical activities in living samples. By labeling a probe protein with a pair of fluorescent proteins, FRET measurement allows to follow a conformational change of the probe sensor to a specific activity. A very exciting challenge is to follow two FRET biosensors at the same time in the same sample and in the same cellular compartment. But the multiplex approach suffers from two limitations: (i) a spectral bleed-through of the first acceptor in the second donor emission band that depends on the concentration of the two biosensors and (ii) the multiple excitation wavelengths which necessitates sequential acquisition that is not adequate to follow fast signal changes in highly dynamic biochemical activities
Document type :
Journal articles
Complete list of metadatas

https://hal-univ-rennes1.archives-ouvertes.fr/hal-01275521
Contributor : Laurent Jonchère <>
Submitted on : Wednesday, February 17, 2016 - 3:48:02 PM
Last modification on : Wednesday, May 16, 2018 - 11:23:52 AM

Links full text

Identifiers

Citation

Claire Demeautis, François Sipieter, Julien Roul, Catherine Chapuis, Sergi Padilla-Parra, et al.. Single Wavelength Excitation Dual Color Flim for Multiplexing Genetically Encoded FRET Biosensors. Biophysical Journal, Biophysical Society, 2016, 110 (3), pp.518a. ⟨10.1016/j.bpj.2015.11.2771⟩. ⟨hal-01275521⟩

Share

Metrics

Record views

328