Identification of CELF1 RNA targets by CLIP-seq in human HeLa cells

Olivier Le Tonquèze (1) , Bernhard Gschloessl (1) , Vincent Legagneux (2) , Luc Paillard (2) , Yann Audic (2)

1 IGDR - Institut de Génétique et Développement de Rennes
2 IGDR - Institut de Génétique et Développement de Rennes

The specific interactions between RNA-binding proteins and their target RNAs are an essential level to control gene expression. By combining ultra-violet cross-linking and immunoprecipitation (CLIP) and massive SoliD sequencing we identified the RNAs bound by the RNA-binding protein CELF1, in human HeLa cells. The CELF1 binding sites deduced from the sequence data allow characterizing specific features of CELF1-RNA association. We present therefore the first map of CELF1 binding sites in human cells.

Mots clés: RNA binding proteins; Homo sapiens; Alternative Splicing; CLIP-seq; CUGBP1

Domaines: Sciences du Vivant [q-bio]

Olivier Le Tonquèze, Bernhard Gschloessl, Vincent Legagneux, Luc Paillard, Yann Audic. Identification of CELF1 RNA targets by CLIP-seq in human HeLa cells. Genomics Data, 2016, 8, pp.97-103. ⟨10.1016/j.gdata.2016.04.009⟩. ⟨hal-01304359⟩