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Multicenter comparison of the etest and EUCAST methods for antifungal susceptibility testing of Candida isolates to micafungin

M.-E. Bougnoux 1 E. Dannaoui 2 I. Accoceberry 3 A. Angoulvant 4, 5 E. Bailly 6 F. Botterel 7, 8 S. Chevrier 9, 10 T. Chouaki 11 M. Cornet 12, 13 F. Dalle 14, 15 A. Datry 16 A. Dupuis 17, 18 A. Fekkar 16 J.P. Gangneux 10 J. Guitard 19 C. Hennequin 20 Y. Le Govic 21 P. Le Pape 22 D. Maubon 13 S. Ranque 23 M. Sautour 16 B. Sendid 24 J. Chandenier 6
Abstract : In vitro susceptibility of 933 Candida isolates, from 16 French hospitals, to micafungin was determined using the Etest in each center. All isolates were then sent to a single center for determination of MICs by the EUCAST reference method. Overall essential agreement between the two tests was 98.5% at ±2 log2 dilutions and 90.2% at ±1 log2 dilutions. Categorical agreement was 98.2%. The Etest is a valuable alternative to EUCAST for the routine determination of micafungin MICs in medical mycology laboratories. Copyright © 2016, American Society for Microbiology. All Rights Reserved.
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Submitted on : Friday, November 4, 2016 - 11:40:39 AM
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M.-E. Bougnoux, E. Dannaoui, I. Accoceberry, A. Angoulvant, E. Bailly, et al.. Multicenter comparison of the etest and EUCAST methods for antifungal susceptibility testing of Candida isolates to micafungin. Antimicrobial Agents and Chemotherapy, American Society for Microbiology, 2016, 60 (8), pp.5088--5091. ⟨10.1128/AAC.00630-16⟩. ⟨hal-01367142⟩

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