Multicenter comparison of the etest and EUCAST methods for antifungal susceptibility testing of Candida isolates to micafungin

M.-E. Bougnoux (1) , E. Dannaoui (2) , I. Accoceberry (3) , A. Angoulvant (4, 5) , E. Bailly (6) , F. Botterel (7, 8) , S. Chevrier (9, 10) , T. Chouaki (11) , M. Cornet (12, 13) , F. Dalle (14, 15) , A. Datry (16) , A. Dupuis (17, 18) , A. Fekkar (16) , J.P. Gangneux (10) , J. Guitard (19) , C. Hennequin (20) , Y. Le Govic (21) , P. Le Pape (22) , D. Maubon (13) , S. Ranque (23) , M. Sautour (16) , B. Sendid (24) , J. Chandenier (6)

1 CHU Necker - Enfants Malades [AP-HP]
2 Hôpital Européen Georges Pompidou
3 CHU Bordeaux [Bordeaux]
4 AP-HP Hôpital Bicêtre (Le Kremlin-Bicêtre)
5 GQE-Le Moulon - Génétique Quantitative et Evolution - Le Moulon (Génétique Végétale)
6 CHRU Tours - Centre Hospitalier Régional Universitaire de Tours
7 Anofel Cryptosporidium National Network
8 Laboratoire de Parasitologie-Mycologie
9 Service de Parasitologie-Mycologie [Rennes]
10 Irset - Institut de recherche en santé, environnement et travail
11 CHU Amiens-Picardie
12 TIMC-IMAG - Techniques de l'Ingénierie Médicale et de la Complexité - Informatique, Mathématiques et Applications, Grenoble - UMR 5525
13 Institut de Biologie et Pathologie
14 Laboratoire de parasitologie mycologie (CHU de Dijon)
15 PAM - Procédés Alimentaires et Microbiologiques
16 CHU Pitié-Salpêtrière [AP-HP]
17 IC2MP - Institut de Chimie des Milieux et Matériaux de Poitiers
18 CHU Poitiers - Centre hospitalier universitaire de Poitiers
19 CIMI - Centre d'Immunologie et de Maladies Infectieuses
20 Groupe Hospitalier Saint Louis - Lariboisière - Fernand Widal [Paris]
21 CHU Angers - Centre Hospitalier Universitaire d'Angers
22 GEOPS - Géosciences Paris Sud
23 APHM - Assistance Publique - Hôpitaux de Marseille
24 Laboratoire de Parasitologie-Mycologie

In vitro susceptibility of 933 Candida isolates, from 16 French hospitals, to micafungin was determined using the Etest in each center. All isolates were then sent to a single center for determination of MICs by the EUCAST reference method. Overall essential agreement between the two tests was 98.5% at ±2 log2 dilutions and 90.2% at ±1 log2 dilutions. Categorical agreement was 98.2%. The Etest is a valuable alternative to EUCAST for the routine determination of micafungin MICs in medical mycology laboratories. Copyright © 2016, American Society for Microbiology. All Rights Reserved.

Domains: Life Sciences [q-bio]; Ecology, environment; Health

M.-E. Bougnoux, E. Dannaoui, I. Accoceberry, A. Angoulvant, E. Bailly, et al.. Multicenter comparison of the etest and EUCAST methods for antifungal susceptibility testing of Candida isolates to micafungin. Antimicrobial Agents and Chemotherapy, 2016, 60 (8), pp.5088--5091. ⟨10.1128/AAC.00630-16⟩. ⟨hal-01367142⟩