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Journal articles
Intranuclear localization of transcription factories and immunoglobulin heavy chain gene alleles during human b-cell maturation
Abstract : Immunoglobulin heavy chain (IGH) gene locus is expressed monoallelically in human B cells. Aim. To study the role of nuclear organization in regulation of the IGH expression during B-cell differentiation. Methods. Immunofluorescence in situ hybridization on 3D-preserved nuclei (3D immuno-FISH). Results. Active RNA polymerase II (Pol II) molecules and the IGH locus were detected in the periphery of the nucleoli at some stages of B-cell differentiation. Conclusions. We observed significant changes in the pattern of distribution of RNA polymerase II in the nucleus during B-cell differentiation, but no preferential co-localization of the productive IGH allele with the transcription factories in the vicinity of the nucleolus and in the nucleoplasm was observed. © 2016 A. Pichugin et al.
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01372752
Contributor : Xavier Chard-Hutchinson <>
Submitted on : Tuesday, September 27, 2016 - 4:01:37 PM
Last modification on : Thursday, January 14, 2021 - 11:20:11 AM
Contributor : Xavier Chard-Hutchinson <>
Submitted on : Tuesday, September 27, 2016 - 4:01:37 PM
Last modification on : Thursday, January 14, 2021 - 11:20:11 AM
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