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Intranuclear localization of transcription factories and immunoglobulin heavy chain gene alleles during human b-cell maturation

Abstract : Immunoglobulin heavy chain (IGH) gene locus is expressed monoallelically in human B cells. Aim. To study the role of nuclear organization in regulation of the IGH expression during B-cell differentiation. Methods. Immunofluorescence in situ hybridization on 3D-preserved nuclei (3D immuno-FISH). Results. Active RNA polymerase II (Pol II) molecules and the IGH locus were detected in the periphery of the nucleoli at some stages of B-cell differentiation. Conclusions. We observed significant changes in the pattern of distribution of RNA polymerase II in the nucleus during B-cell differentiation, but no preferential co-localization of the productive IGH allele with the transcription factories in the vicinity of the nucleolus and in the nucleoplasm was observed. © 2016 A. Pichugin et al.
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https://hal-univ-rennes1.archives-ouvertes.fr/hal-01372752
Contributor : Xavier Chard-Hutchinson <>
Submitted on : Tuesday, September 27, 2016 - 4:01:37 PM
Last modification on : Wednesday, September 16, 2020 - 5:26:19 PM

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A.V. Pichugin, O.V. Iarovaia, I.V. Sklyar, G. Lacombe, S.V. Razin, et al.. Intranuclear localization of transcription factories and immunoglobulin heavy chain gene alleles during human b-cell maturation. Biopolymers and Cell, Institute of Molecular Biology and Genetics of the NAS of Ukraine, 2016, 32 (3), pp.179--183. ⟨10.7124/bc.00091C⟩. ⟨hal-01372752⟩

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