Intranuclear localization of transcription factories and immunoglobulin heavy chain gene alleles during human b-cell maturation - Archive ouverte HAL Access content directly
Journal Articles Biopolymers and Cell Year : 2016

Intranuclear localization of transcription factories and immunoglobulin heavy chain gene alleles during human b-cell maturation

Abstract

Immunoglobulin heavy chain (IGH) gene locus is expressed monoallelically in human B cells. Aim. To study the role of nuclear organization in regulation of the IGH expression during B-cell differentiation. Methods. Immunofluorescence in situ hybridization on 3D-preserved nuclei (3D immuno-FISH). Results. Active RNA polymerase II (Pol II) molecules and the IGH locus were detected in the periphery of the nucleoli at some stages of B-cell differentiation. Conclusions. We observed significant changes in the pattern of distribution of RNA polymerase II in the nucleus during B-cell differentiation, but no preferential co-localization of the productive IGH allele with the transcription factories in the vicinity of the nucleolus and in the nucleoplasm was observed. © 2016 A. Pichugin et al.

Dates and versions

hal-01372752 , version 1 (27-09-2016)

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A.V. Pichugin, O.V. Iarovaia, I.V. Sklyar, G. Lacombe, S.V. Razin, et al.. Intranuclear localization of transcription factories and immunoglobulin heavy chain gene alleles during human b-cell maturation. Biopolymers and Cell, 2016, 32 (3), pp.179--183. ⟨10.7124/bc.00091C⟩. ⟨hal-01372752⟩
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