Intranuclear localization of transcription factories and immunoglobulin heavy chain gene alleles during human b-cell maturation

A.V. Pichugin (1) , O.V. Iarovaia (1) , I.V. Sklyar (1) , G. Lacombe (2) , S.V. Razin , Thierry Fest (2) , M. Lipinski (1) , Y.S. Vassetzky (1)

1 UMR8126 - Signalisation, noyaux et innovations en cancérologie
2 MiCa - Microenvironnement et cancer

Immunoglobulin heavy chain (IGH) gene locus is expressed monoallelically in human B cells. Aim. To study the role of nuclear organization in regulation of the IGH expression during B-cell differentiation. Methods. Immunofluorescence in situ hybridization on 3D-preserved nuclei (3D immuno-FISH). Results. Active RNA polymerase II (Pol II) molecules and the IGH locus were detected in the periphery of the nucleoli at some stages of B-cell differentiation. Conclusions. We observed significant changes in the pattern of distribution of RNA polymerase II in the nucleus during B-cell differentiation, but no preferential co-localization of the productive IGH allele with the transcription factories in the vicinity of the nucleolus and in the nucleoplasm was observed. © 2016 A. Pichugin et al.

Domains: Life Sciences [q-bio]; Cancer

A.V. Pichugin, O.V. Iarovaia, I.V. Sklyar, G. Lacombe, S.V. Razin, et al.. Intranuclear localization of transcription factories and immunoglobulin heavy chain gene alleles during human b-cell maturation. Biopolymers and Cell, 2016, 32 (3), pp.179--183. ⟨10.7124/bc.00091C⟩. ⟨hal-01372752⟩