Spatial mapping of lichen specialized metabolites using LDI-MSI: Chemical ecology issues for Ophioparma ventosa

P. Le Pogam (1, 2) , B. Legouin (1) , A. Geairon (3) , H. Rogniaux (3) , Françoise Lohézic-Le Dévéhat (1) , W. Obermayer , J. Boustie (1) , Anne-Cécile Le Lamer (1, 4)

1 ISCR - Institut des Sciences Chimiques de Rennes
2 IETR - Institut d'Électronique et des Technologies du numéRique
3 BIA - Unité de recherche sur les Biopolymères, Interactions Assemblages
4 PHARMA-DEV - Pharmacochimie et Biologie pour le Développement

Imaging mass spectrometry techniques have become a powerful strategy to assess the spatial distribution of metabolites in biological systems. Based on auto-ionisability of lichen metabolites using LDI-MS, we herein image the distribution of major secondary metabolites (specialized metabolites) from the lichen Ophioparma ventosa by LDI-MSI (Mass Spectrometry Imaging). Such technologies offer tremendous opportunities to discuss the role of natural products through spatial mapping, their distribution patterns being consistent with previous chemical ecology reports. A special attention was dedicated to miriquidic acid, an unexpected molecule we first reported in Ophioparma ventosa. The analytical strategy presented herein offers new perspectives to access the sharp distribution of lichen metabolites from regular razor blade-sectioned slices. © The Author(s) 2016.

Domaines: Réseaux et télécommunications [cs.NI]; Electronique

P. Le Pogam, B. Legouin, A. Geairon, H. Rogniaux, Françoise Lohézic-Le Dévéhat, et al.. Spatial mapping of lichen specialized metabolites using LDI-MSI: Chemical ecology issues for Ophioparma ventosa. Scientific Reports, 2016, 6 (1), pp.37807. ⟨10.1038/srep37807⟩. ⟨hal-01416316⟩