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Journal articles
Monitoring Calcium Oscillations in Fertilized Mouse Eggs
Abstract : In mammalian species, including human, fertilization is characterized by the triggering of long-lasting calcium (Ca(2+)) oscillations in the egg cytoplasm. The monitoring of these Ca(2+) oscillations is a valuable technique to demonstrate that fertilization has occurred, to study egg activation events elicited downstream of the Ca(2+) signal, as well as to evaluate sperm quality. This chapter describes our protocol to monitor sperm-induced Ca(2+) oscillations in mouse eggs, using fluorescence microscopy techniques and the Fura-2-AM ratiometric Ca(2+) indicator.
Cited literature [21 references]
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01439381
Contributor : Laurent Jonchère Connect in order to contact the contributor
Submitted on : Friday, March 24, 2017 - 10:22:52 AM
Last modification on : Thursday, January 14, 2021 - 11:21:01 AM
Long-term archiving on: : Sunday, June 25, 2017 - 12:46:20 PM
Contributor : Laurent Jonchère Connect in order to contact the contributor
Submitted on : Friday, March 24, 2017 - 10:22:52 AM
Last modification on : Thursday, January 14, 2021 - 11:21:01 AM
Long-term archiving on: : Sunday, June 25, 2017 - 12:46:20 PM
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