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CD95 Stimulation with CD95L and DISC Analysis

Abstract

CD95 and its ligand CD95L play a major role in immune surveillance and homeostasis. CD95L is expressed by activated T lymphocytes and NK cells to induce apoptosis in cancer and virus-infected cells. The goal of this chapter is to describe a method used to immunoprecipitate CD95 and analyze its associated protein complex in cells stimulated with a cytotoxic CD95L (i.e., Ig-CD95L).
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Dates and versions

hal-01447229 , version 1 (26-01-2017)

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Matthieu Le Gallo, Patrick Legembre. CD95 Stimulation with CD95L and DISC Analysis. CD95: Methods and Protocols, 1557, pp.11-18, 2017, Methods in Molecular Biology, ⟨10.1007/978-1-4939-6780-3_2⟩. ⟨hal-01447229⟩
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