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Study of the CD95-Mediated Non-apoptotic Signaling Pathway: PI3K

Abstract : CD95 is a plasma membrane receptor that belongs to the TNF receptor family (Itoh and Nagata, J Biol Chem 268(15):10932-10937, 1993; Trauth et al., Science 245(4915):301-305, 1989). Accumulating evidence indicate that this so-called death receptor can also trigger non-apoptotic signaling pathways promoting inflammation and oncogenesis (Barnhart et al., Embo J 23(15):3175-3185, 2004; Chen et al., Nature 465(7297):492-496, 2010; Legembre et al., Cell Cycle 3(10):1235-1239, 2004; Legembre et al., EMBO Rep 5(11):1084-1089, 2004; Malleter et al., Cancer Res 73(22):6711-6721, 2013; Tauzin et al., PLoS Biol 9(6):e1001090, 2011). We and others demonstrated that CD95 implements the PI3K signaling pathway through the formation of a molecular complex designated Motility Inducing Signaling Complex (MISC) contributing to cell survival, growth, proliferation, differentiation and motility (Malleter et al., Cancer Res 73(22):6711-6721, 2013; Tauzin et al., PLoS Biol 9(6):e1001090, 2011; Kleber et al., Cancer Cell 13(3):235-248, 2008). This chapter describes how to immunoprecipitate CD95 to characterize MISC involved in PI3K activation.
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https://hal-univ-rennes1.archives-ouvertes.fr/hal-01447230
Contributor : Laurent Jonchère <>
Submitted on : Thursday, January 26, 2017 - 4:44:31 PM
Last modification on : Monday, March 25, 2019 - 11:49:56 AM

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Amelie Fouque, Patrick Legembre. Study of the CD95-Mediated Non-apoptotic Signaling Pathway: PI3K. CD95: Methods and Protocols, 1557, pp.103-110, 2017, Methods in Molecular Biology, ⟨10.1007/978-1-4939-6780-3_10⟩. ⟨hal-01447230⟩

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