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A misleading false-negative result of Pneumocystis real-time PCR assay due to a rare punctual mutation: A French multicenter study.

Abstract : This article describes a previously unreported mutation at position 210 (C210T) of the mitochondrial large subunit ribosomal RNA (mtLSUrRNA) gene of Pneumocystis jirovecii, which led to a false-negative result of a real-time polymerase chain reaction (PCR) assay. Since the aforementioned real-time PCR assay is widely used in France, a French multicenter study was conducted to estimate the mutation frequency and its potential impact on the routine diagnosis of Pneumocystis pneumonia (PCP). Through analysis of data obtained from eight centers, the mutation frequency was estimated at 0.28%. This low frequency should not call into question the routine use of this PCR assay. Nonetheless, the occurrence of the false-negative PCR result provides arguments for maintaining microscopic techniques combined to PCR assays to achieve PCP diagnosis.
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https://hal-univ-rennes1.archives-ouvertes.fr/hal-01477200
Contributor : Xavier Chard-Hutchinson <>
Submitted on : Monday, February 27, 2017 - 10:38:20 AM
Last modification on : Wednesday, October 14, 2020 - 3:11:40 AM

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Solène Le Gal, Florence Robert-Gangneux, Yann Pepino, Sorya Belaz, Céline Damiani, et al.. A misleading false-negative result of Pneumocystis real-time PCR assay due to a rare punctual mutation: A French multicenter study.. Medical Mycology, Oxford University Press, 2017, 55 (2), pp.180-184. ⟨10.1093/mmy/myw051⟩. ⟨hal-01477200⟩

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