Multiplexing PKA and ERK1&2 kinases FRET biosensors in living cells using single excitation wavelength dual colour FLIM

Claire Demeautis (1) , Francois Sipieter (2, 3) , Julien Roul (1) , Catherine Chapuis (1) , Sergi Padilla-Parra , Franck B. Riquet (4, 3) , Marc Tramier (1)

1 IGDR - Institut de Génétique et Développement de Rennes
2 PhLAM - Laboratoire de Physique des Lasers, Atomes et Molécules - UMR 8523
3 UGENT - Universiteit Gent = Ghent University
4 UGSF - Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576

Keywords: cyclic-amp; a activity; map kinase; large stokes shift; fluorescent protein; erk activity; signal-regulated kinase; activated protein-kinase; growth-factor receptor; resonance energy-transfer

Domains: Life Sciences [q-bio]; Genetics

Claire Demeautis, Francois Sipieter, Julien Roul, Catherine Chapuis, Sergi Padilla-Parra, et al.. Multiplexing PKA and ERK1&2 kinases FRET biosensors in living cells using single excitation wavelength dual colour FLIM. Scientific Reports, 2017, 7 (1), pp.41026. ⟨10.1038/srep41026⟩. ⟨hal-01478382⟩