Minimal residual disease detection in tunisian b-acute lymphoblastic leukemia based on immunoglobulin gene rearrangements

Abstract : IGH gene rearrangement and IGK-Kde gene deletion can be used as molecular markers for the assessment of B lineage acute lymphoblastic leukemia (B-ALL). Minimal residual disease detected based on those markers is currently the most reliable prognosis factor in B-ALL. The aim of this study was to use clonal IGH/IGK-Kde gene rearrangements to confirm B-ALL diagnosis and to evaluate the treatment outcome of Tunisian leukemic patients by monitoring the minimal residual disease (MRD) after induction chemotherapy. Seventeen consecutive newly diagnosed B-ALL patients were investigated by multiplex PCR assay and real time quantitative PCR according to BIOMED 2 conditions. The vast majority of clonal VH-JH rearrangements included VH3 gene. For IGK deletion, clonal VK1f/6-Kde recombinations were mainly identified. These rearrangements were quantified to follow-up seven B-ALL after induction using patient-specific ASO. Four patients had an undetectable level of MRD with a sensitivity of up to 10-5. This molecular approach allowed identification of prognosis risk group and adequate therapeutic decision. The IGK-Kde and IGH gene rearrangements might be used for diagnosis and MRD monitoring of B-ALL, introduced for the first time in Tunisian laboratories.
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https://hal-univ-rennes1.archives-ouvertes.fr/hal-01485940
Contributor : Xavier Chard-Hutchinson <>
Submitted on : Thursday, March 9, 2017 - 3:21:59 PM
Last modification on : Monday, November 18, 2019 - 1:14:28 PM

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S. Besbes, W.S. Hamadou, M.L. Boulland, Y.B. Youssef, B. Achour, et al.. Minimal residual disease detection in tunisian b-acute lymphoblastic leukemia based on immunoglobulin gene rearrangements. Brazilian Journal of Medical and Biological Research, Associação Brasileira de Divulgação Científica, 2017, 50 (1), pp.e5426. ⟨10.1590/1414-431X20165426⟩. ⟨hal-01485940⟩

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