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Journal articles
The AID-Cre-ERT2 Model: A Tool for Monitoring B Cell Immune Responses and Generating Selective Hybridomas
Abstract : Expression of activation-induced cytidine deaminase (AID) is the hallmark of B cells engaged in an immune response in germinal centers. We designed an inducible fate-mapping reporter mouse in which AID-expressing B cells could be timely and irreversibly marked, by knockin at the Aicda locus of a tamoxifen-inducible Cre recombinase. This mouse model allows notably for the long-term follow-up of memory B cells and plasma cells engaged in an immune response. We describe here a protocol to generate hybridomas from small memory subsets that can be easily traced and identified in this mouse line through Cre-activated fluorescent reporters.
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01560459
Contributor : Laurent Jonchère <>
Submitted on : Tuesday, July 11, 2017 - 3:37:58 PM
Last modification on : Thursday, January 14, 2021 - 11:21:01 AM
Contributor : Laurent Jonchère <>
Submitted on : Tuesday, July 11, 2017 - 3:37:58 PM
Last modification on : Thursday, January 14, 2021 - 11:21:01 AM