Evaluation of whole-blood conservation reagents for Hematoflow-based WBC differential count: Unsatisfactory results

Orianne Wagner-Ballon (1) , Bouchra Badaoui (1) , Audrey Brignoli (1) , Jérôme Le Priol (2) , Mikael Roussel (3, 2)

1 CHU Henri Mondor [Créteil]
2 CHU Pontchaillou [Rennes]
3 MICMAC - Microenvironment, Cell Differentiation, Immunology and Cancer

Background:As conservation of whole blood samples undergoing white blood cell (WBC) differential performed by flow cytometry (Hematoflow) is needed, we evaluated the effects of two commercially available fixatives, namely TransfixTM and Streck Cell PreservativeTM.Methods:We focused on 15 normal samples and on 13 various pathological samples. We compared the two fixatives and cold- or room- temperature effects on various parameters provided by the Hematoflow system.Results:We observed that, even after 2 hours of sample treatment, the conservative methods led to significant modifications of the cell percentages due to substantial variations of the epitope expression.Conclusion:None of the different conservation methods is really reliable for WBC differential performed by flow cytometry and thus samples should be analyzed promptly or stored at 4°C.

Keywords: Flow Cytometry; Leukocyte differential; blood preservation; Transfix; Streck Cell Preservative

Domains: Life Sciences [q-bio]; Cancer

Orianne Wagner-Ballon, Bouchra Badaoui, Audrey Brignoli, Jérôme Le Priol, Mikael Roussel. Evaluation of whole-blood conservation reagents for Hematoflow-based WBC differential count: Unsatisfactory results. International Journal of Laboratory Hematology, 2017, 39 (5), pp.e127-e131. ⟨10.1111/ijlh.12697⟩. ⟨hal-01616400⟩