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Journal articles
14-3-3 regulation of Ncd reveals a new mechanism for targeting proteins to the spindle in oocytes
Abstract : The meiotic spindle is formed without centrosomes in a large volume of oocytes. Local activation of crucial spindle proteins around chromosomes is important for formation and maintenance of a bipolar spindle in oocytes. We found that phosphodocking 14-3-3 proteins stabilize spindle bipolarity in Drosophila melanogaster oocytes. A critical 14-3-3 target is the minus end-directed motor Ncd (human HSET; kinesin-14), which has well-documented roles in stabilizing a bipolar spindle in oocytes. Phospho docking by 14-3-3 inhibits the microtubule binding activity of the nonmotor Ncd tail. Further phosphorylation by Aurora B kinase can release Ncd from this inhibitory effect of 14-3-3. As Aurora B localizes to chromosomes and spindles, 14-3-3 facilitates specific association of Ncd with spindle microtubules by preventing Ncd from binding to nonspindle microtubules in oocytes. Therefore, 14-3-3 translates a spatial cue provided by Aurora B to target Ncd selectively to the spindle within the large volume of oocytes.
Cited literature [51 references]
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01616455
Contributor : Laurent Jonchère Connect in order to contact the contributor
Submitted on : Thursday, October 4, 2018 - 4:25:44 PM
Last modification on : Monday, July 5, 2021 - 3:40:14 PM
Long-term archiving on: : Saturday, January 5, 2019 - 5:13:20 PM
Contributor : Laurent Jonchère Connect in order to contact the contributor
Submitted on : Thursday, October 4, 2018 - 4:25:44 PM
Last modification on : Monday, July 5, 2021 - 3:40:14 PM
Long-term archiving on: : Saturday, January 5, 2019 - 5:13:20 PM
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JCB_201704120.pdf
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