Thymoquinone prevents endoplasmic reticulum stress and mitochondria-induced apoptosis in a rat model of partial hepatic warm ischemia reperfusion

Ahlem Bouhlel Ismail Ben Mosbah 1, 2 Najet Hadj Abdallah Catherine Ribault 3 Roselyne Viel 4 Saber Mannaï Anne Corlu 3 Hassen Ben Abdennebi
4 H2P2 - H2P2 - Histo Pathologie Hight Precision
Biosit : Biologie - Santé - Innovation Technologique - Structure Fédérative de Recherche en Biologie et Santé de Rennes , UR1 - Université de Rennes 1
Abstract : This study was undertaken to evaluate the protective effect of thymoquinone (TQ), the bioactive compound of Nigella sativa seeds, against warm ischemia-reperfusion (I/R) injury in liver. Rats were given an oral administration of a vehicle solution (sham group) or TQ at the appropriate dose (10, 20, 30 and 40mg/kg) for ten days consecutively. Following, they were subjected to 60min of partial hepatic ischemia followed by 24h of reperfusion. .Transaminase activities, histopathological changes, TNFα and antioxidant parameters were evaluated. Also, endoplasmic reticulum (ER) stress, mitochondrial damage and apoptosis were studied. In addition, ERK and P38 phosphorylation was determined by Western blot technique. We found that TQ at 30mg/kg is the effective dose to protect rat liver against I/R injury. Moreover, 30mg/kg of TQ prevented histological damages, inflammation and oxidative stress. Interestingly, it decreased the expression of ER stress parameters including GRP78, CHOP and caspase-12. In parallel, it improved mitochondrial function and attenuated the expression of apoptotic parameters. Furthermore, TQ significantly enhanced ERK and P38 phosphorylation. In conclusion, we demonstrated the potential of TQ to protect the rat liver against I/R injury through the prevention of ER stress and mitochondrial dysfunction. These effects implicate the prevention of oxidative stress.
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https://hal-univ-rennes1.archives-ouvertes.fr/hal-01618032
Contributeur : Laurent Jonchère <>
Soumis le : mardi 17 octobre 2017 - 13:50:02
Dernière modification le : mercredi 16 mai 2018 - 11:24:11

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Ahlem Bouhlel, Ismail Ben Mosbah, Najet Hadj Abdallah, Catherine Ribault, Roselyne Viel, et al.. Thymoquinone prevents endoplasmic reticulum stress and mitochondria-induced apoptosis in a rat model of partial hepatic warm ischemia reperfusion. Biomedicine & Pharmacotherapy, 2017, 94, pp.964-973. 〈10.1016/j.biopha.2017.08.018〉. 〈hal-01618032〉

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