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Journal articles
A Genetic Tool to Quantify trans-Translation Activity in Vivo
Abstract : In bacteria, trans-translation is the main quality control mechanism for rescuing ribosomes arrested during translation. This key process is universally conserved and plays a critical role in the viability and virulence of many pathogens. We developed a reliable in vivo double-fluorescence reporter system for the simultaneous quantification of both trans-translation and the associated proteolysis activities in bacteria. The assay was validated using mutant bacteria lacking tmRNA, SmpB, and the ClpP protease. Both antisense tmRNA-binding RNA and a peptide mimicking the SmpB C-terminal tail proved to be potent inhibitors of trans-translation in vivo. The double-fluorescent reporter was also tested with KKL-35, an oxadiazole derivative that is supposed to be a promising trans-translation inhibitor, and it surprisingly turns out that trans-translation is not the only target of KKL-35 in vivo.
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01659526
Contributor : Xavier Chard-Hutchinson <>
Submitted on : Friday, December 8, 2017 - 2:50:25 PM
Last modification on : Thursday, February 27, 2020 - 4:45:55 PM
Contributor : Xavier Chard-Hutchinson <>
Submitted on : Friday, December 8, 2017 - 2:50:25 PM
Last modification on : Thursday, February 27, 2020 - 4:45:55 PM