Validating Missing Proteins in Human Sperm Cells by Targeted Mass-Spectrometry- and Antibody-based Methods

Christine Carapito (1) , Paula Duek (2) , Charlotte Macron (1) , Marine Seffals (3) , Karine Rondel (4) , François Delalande (1) , Cecilia Lindskog (5) , Thomas Freour (6, 7) , Yves Vandenbrouck (8, 9, 10) , Lydie Lane (11) , Charles Pineau (4)

1 IPHC - Institut Pluridisciplinaire Hubert Curien
2 SIB - Swiss Institute of Bioinformatics [Genève]
3 H2P2 - H2P2 - Histo Pathologie Hight Precision
4 Irset - Institut de recherche en santé, environnement et travail
5 Uppsala University
6 CHU Nantes - Centre hospitalier universitaire de Nantes
7 U1064 Inserm - CRTI - Centre de Recherche en Transplantation et Immunologie
8 CEA - Commissariat à l'énergie atomique et aux énergies alternatives
9 BGE - UMR S1038 - Laboratoire de Biologie à Grande Échelle
10 UGA [2016-2019] - Université Grenoble Alpes [2016-2019]
11 SIB - Swiss Institute of Bioinformatics [Lausanne]

Keywords: human proteome project; spermatozoon; missing proteins; parallel reaction monitoring; targeted proteomics; immunohistochemistry; immunocytochemistry; bioinformatics; data mining

Domains: Life Sciences [q-bio]

Christine Carapito, Paula Duek, Charlotte Macron, Marine Seffals, Karine Rondel, et al.. Validating Missing Proteins in Human Sperm Cells by Targeted Mass-Spectrometry- and Antibody-based Methods. Journal of Proteome Research, 2017, 16 (12), pp.4340-4351. ⟨10.1021/acs.jproteome.7b00374⟩. ⟨hal-01681656⟩